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ZFIN ID: ZDB-FIG-090527-2
Vitorino et al., 2009 - Vsx2 in the zebrafish retina: restricted lineages through derepression. Neural Development   4:14 Full text @ Neural Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: 26+ somites to Day 4

Fig. 1 Vsx2 expression in the zebrafish retina. (A-G) Cryosections through the central retina of zebrafish embryos counterstained with DAPI (blue). Developmental stages are indicated by hours post-fertilization (hpf) at 28.5°C. (A, B) vsx2 in situ hybridization at 24 hpf (A) and 72 hpf (B). (C, D) Tg(vsx2:GFP) shows green fluorescent protein (GFP) expression in retinal progenitor cells (C) and in cells of the outer part of the INL (D). Arrows indicate vsx2 RNA (B) and Vsx2:GFP (D) expression in the ciliary margin (CM). (E-G) Tg(vsx2:GFP) expressing retinas labelled with the bipolar cell marker protein kinase C (PKC) at 80 hpf (E) and the two Müller cell markers glutamine synthetase (GS) (F) and Sox2 (G). No Vsx2:GFP-positive cells co-localise with the bipolar marker at 80 hpf (E). Müller cells expressing GS and Sox2 are also Vsx2:GFP-positive ((F″, G″) asterisks). GCL, ganglion cell layer; INL, inner nuclear layer; ipl, inner plexiform layer; L, lens; ONL, outer nuclear layer; opl, outer plexiform layer. Scale bars: (A-D) 100 μm; (E-G) 40 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP nns1Tg standard conditions 26+ somites optic cup IFL
Protruding-mouth ciliary marginal zone IFL
Protruding-mouth retinal inner nuclear layer Muller cell IFL
Protruding-mouth retinal inner nuclear layer retinal bipolar neuron IFL
Protruding-mouth retinal outer nuclear layer IFL
Day 4 ciliary marginal zone IFL
Day 4 retinal inner nuclear layer retinal bipolar neuron IFL
sox2 Ab1-sox2 nns1Tg standard conditions Protruding-mouth retinal inner nuclear layer Muller cell IHC
vsx2 WT standard conditions Prim-5 optic cup ISH
Protruding-mouth ciliary marginal zone ISH
Protruding-mouth retina ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Neural Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Neural Dev.