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ZFIN ID: ZDB-FIG-090527-2
Vitorino et al., 2009 - Vsx2 in the zebrafish retina: restricted lineages through derepression. Neural Development   4:14 Full text @ Neural Dev.
Anatomical Terms:
Stage Range: 26+ somites to Day 4

Fig. 1 Vsx2 expression in the zebrafish retina. (A-G) Cryosections through the central retina of zebrafish embryos counterstained with DAPI (blue). Developmental stages are indicated by hours post-fertilization (hpf) at 28.5°C. (A, B) vsx2 in situ hybridization at 24 hpf (A) and 72 hpf (B). (C, D) Tg(vsx2:GFP) shows green fluorescent protein (GFP) expression in retinal progenitor cells (C) and in cells of the outer part of the INL (D). Arrows indicate vsx2 RNA (B) and Vsx2:GFP (D) expression in the ciliary margin (CM). (E-G) Tg(vsx2:GFP) expressing retinas labelled with the bipolar cell marker protein kinase C (PKC) at 80 hpf (E) and the two Müller cell markers glutamine synthetase (GS) (F) and Sox2 (G). No Vsx2:GFP-positive cells co-localise with the bipolar marker at 80 hpf (E). Müller cells expressing GS and Sox2 are also Vsx2:GFP-positive ((F″, G″) asterisks). GCL, ganglion cell layer; INL, inner nuclear layer; ipl, inner plexiform layer; L, lens; ONL, outer nuclear layer; opl, outer plexiform layer. Scale bars: (A-D) 100 μm; (E-G) 40 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP nns1Tg standard conditions 26+ somites optic cup IFL
Protruding-mouth ciliary marginal zone IFL
Protruding-mouth retinal inner nuclear layer Muller cell IFL
Protruding-mouth retinal inner nuclear layer retinal bipolar neuron IFL
Protruding-mouth retinal outer nuclear layer IFL
Day 4 ciliary marginal zone IFL
Day 4 retinal inner nuclear layer retinal bipolar neuron IFL
sox2 Ab1-sox2 nns1Tg standard conditions Protruding-mouth retinal inner nuclear layer Muller cell IHC
vsx2 WT standard conditions Prim-5 optic cup ISH
Protruding-mouth ciliary marginal zone ISH
Protruding-mouth retina ISH
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal Neural Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Neural Dev.