ZFIN ID: ZDB-FIG-090320-47
Ochi et al., 2009 - Lbx2 regulates formation of myofibrils. BMC Developmental Biology   9:13 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes: acta1a, mybpc1, myhz1.1, myhz2, mylpfa (all 10) expand
Fish: WT, WT + MO2-lbx2
Knockdown Reagent: MO2-lbx2
Anatomical Terms: fast muscle cell striated muscle myosin thick filament, fast muscle cell striated muscle thin filament, slow muscle cell striated muscle myosin thick filament, slow muscle cell striated muscle thin filament
Stage: Prim-5

Fig. 6 Expression of thin and thick myofilament genes is downregulated by interference with Lbx2 activity. A-J: Analysis of myofilament gene expression. Expression of acta1 (A), tnnt1 (B), tpma (C), tnnt3b (D), tnnc (E), myhz1 (F), mybpc1 (G), smyhc (H), myhz2 (I), mylz2 (J) in control, lbx2-MO injected, lbx2 mRNA injected, and Engrailed suppresser domain fused lbx2 (EnR-lbx2) mRNA injected embryos. Expression of acta1 (A, ctrl: 28/28, lbx2-MO: 0/33, lbx2-mRNA: 9/10, EnR-lbx2 mRNA: 8/8, numerator indicates number of embryos with normal expression, and denominator indicates number of examined embryos), tnnt1 (B, ctrl: 19/19, lbx2-MO: 7/22, lbx2-mRNA: 12/12, EnR-lbx2 mRNA: 17/17), tpma (C, ctrl: 16/16, lbx2-MO: 2/20, lbx2-mRNA: 13/14, EnR-lbx2 mRNA: 10/11) and tnnt3b (D, ctrl: 13/13, lbx2-MO: 0/7, lbx2-mRNA: 7/7, EnR-lbx2 mRNA: 4/4) is reduced by lbx2-MO, but not tnnc (E, ctrl: 17/17, lbx2-MO: 18/22, lbx2-mRNA: 10/10, EnR-lbx2 mRNA: 16/16). Expression of myhz1 (F, ctrl: 25/25, lbx2-MO: 3/16, lbx2-mRNA: 7/8, EnR-lbx2 mRNA: 8/8), mybpc1 (G, ctrl: 15/15, lbx2-MO: 10/20, lbx2-mRNA: 13/14, EnR-lbx2 mRNA: 5/8) and myhz2 (I, ctrl: 17/17, lbx2-MO: 0/5, lbx2-mRNA: 9/9, EnR-lbx2 mRNA: 8/8) is reduced by lbx2-MO, but smyhc (H, ctrl: 36/36, lbx2-MO: 24/29, lbx2-mRNA: 8/8, EnR-lbx2 mRNA: 12/12) and mylz2 are unaltered (J, ctrl: 15/15, lbx2-MO: 14/15, lbx2-mRNA: 10/10, EnR-lbx2 mRNA: 7/7). No obvious difference can be detected between embryos injected with lbx2 mRNA and embryos injected with EnR-lbx2 mRNA. (A-J) Whole mounts, lateral views, rostral toward the left, dorsal toward the top. Scale bar: 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
acta1a WT control Prim-5 fast muscle cell striated muscle thin filament ISH
Prim-5 slow muscle cell striated muscle thin filament ISH
WT + MO2-lbx2 standard conditions Prim-5 Not Detected fast muscle cell striated muscle thin filament ISH
Prim-5 Not Detected slow muscle cell striated muscle thin filament ISH
mybpc1 WT control Prim-5 slow muscle cell striated muscle myosin thick filament ISH
WT + MO2-lbx2 standard conditions Prim-5 Not Detected slow muscle cell striated muscle myosin thick filament ISH
myhz1.1 WT control Prim-5 fast muscle cell striated muscle myosin thick filament ISH
Prim-5 slow muscle cell striated muscle myosin thick filament ISH
WT + MO2-lbx2 standard conditions Prim-5 Not Detected fast muscle cell striated muscle myosin thick filament ISH
Prim-5 Not Detected slow muscle cell striated muscle myosin thick filament ISH
myhz2 WT control Prim-5 fast muscle cell striated muscle myosin thick filament ISH
WT + MO2-lbx2 standard conditions Prim-5 Not Detected fast muscle cell striated muscle myosin thick filament ISH
mylpfa WT control Prim-5 fast muscle cell striated muscle myosin thick filament ISH
WT + MO2-lbx2 standard conditions Prim-5 fast muscle cell striated muscle myosin thick filament ISH
smyhc1 WT control Prim-5 slow muscle cell striated muscle myosin thick filament ISH
WT + MO2-lbx2 standard conditions Prim-5 slow muscle cell striated muscle myosin thick filament ISH
tnnc2 WT control Prim-5 fast muscle cell striated muscle thin filament ISH
WT + MO2-lbx2 standard conditions Prim-5 Not Detected fast muscle cell striated muscle thin filament ISH
tnnt1 WT control Prim-5 slow muscle cell striated muscle thin filament ISH
WT + MO2-lbx2 standard conditions Prim-5 Not Detected slow muscle cell striated muscle thin filament ISH
tnnt3b WT control Prim-5 fast muscle cell striated muscle thin filament ISH
WT + MO2-lbx2 standard conditions Prim-5 Not Detected fast muscle cell striated muscle thin filament ISH
tpma WT control Prim-5 fast muscle cell striated muscle thin filament ISH
WT + MO2-lbx2 standard conditions Prim-5 Not Detected fast muscle cell striated muscle thin filament ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.