ZFIN ID: ZDB-FIG-090320-48
Ochi et al., 2009 - Lbx2 regulates formation of myofibrils. BMC Developmental Biology   9:13 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes: acta1a, mef2ca, mybpc1, myog
Fish: WT, WT + MO2-lbx2
Knockdown Reagent: MO2-lbx2
Anatomical Terms: fast muscle cell striated muscle thin filament, muscle cell, slow muscle cell striated muscle myosin thick filament, slow muscle cell striated muscle thin filament
Stage: Prim-5

Fig. 7 The Engrailed domain of Lbx2 is required for myofilament gene expression. A-H: Expression of acta1 (A-D) and mybpc1 (E-H). Embryos express acta1at 24 hpf (A: 93% with normal expression, n = 15). Injection of lbx2-MO results in decreased acta1 expression (B: 20% with normal expression, n = 20), whereas acta1 expression partially recovers in embryos injected with lbx2-MO + lbx2 mRNA (C: 42% with normal expression, n = 50). In contrast, embryos injected with lbx2-MO + lbx Δ En mRNA fail to express acta1 (D: 14% with normal expression, n = 37). mybpc1 (E: 94%, n = 18; F: 43%, n = 23; G: 81%, n = 31; H: 42%, n = 38). I-P: Expression of mef2ca (I-L) and myogenin (M-P). Embryos express mef2ca at 24 hpf (I: n = 11). Injection of lbx2-MO results in increased mef2ca expression (J: 72% with increased expression, n = 11), whereas mef2ca expression partially recovers in embryos injected with lbx2-MO + lbx2 mRNA (K: 21% with increased expression, n = 19). In contrast, embryos injected with lbx2-MO + lbx Δ En mRNA fail to express mef2ca (L: 61% with increased expression, n = 21). myogenin (M: n = 7, N: 78% with increased expression, n = 9, O: 23%, n = 13, P: 67%, n = 14). (A-P) Whole mounts, lateral views, rostral toward the left, dorsal toward the top. Scale bar: 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
acta1a WT control Prim-5 fast muscle cell striated muscle thin filament ISH
Prim-5 slow muscle cell striated muscle thin filament ISH
WT + MO2-lbx2 standard conditions Prim-5 Not Detected fast muscle cell striated muscle thin filament ISH
Prim-5 Not Detected slow muscle cell striated muscle thin filament ISH
mef2ca WT control Prim-5 muscle cell ISH
WT + MO2-lbx2 standard conditions Prim-5 muscle cell ISH
mybpc1 WT control Prim-5 slow muscle cell striated muscle myosin thick filament ISH
WT + MO2-lbx2 standard conditions Prim-5 Not Detected slow muscle cell striated muscle myosin thick filament ISH
myog WT control Prim-5 muscle cell ISH
WT + MO2-lbx2 standard conditions Prim-5 muscle cell ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.