ZFIN ID: ZDB-FIG-090320-43
Ochi et al., 2009 - Lbx2 regulates formation of myofibrils. BMC Developmental Biology   9:13 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes: fgf8a, myod1
Fish: WT, WT + MO1-lbx1a, WT + MO2-lbx2
Knockdown Reagents: MO1-lbx1a, MO2-lbx2
Anatomical Terms: ectoderm, hyoid muscle
Stage: Long-pec

Fig. 2 Lbx2 and Lbx1b function in hypaxial muscle development. A: Splice donor MOs against lbx2 or lbx1b inhibit correct splicing of lbx2 or lbx1b, respectively. RT-PCR was performed using bud stage (lbx2) or segmentation stage (lbx1b) embryos. Spliced bands and unspliced bands in the lbx-splice donor-MO lane indicate aberrantly spliced message increases and correctly spliced message decreases. Arrows indicate primers. En: Engrailed domain, HD: homeodomain. Arrows indicate specific primers for amplification of correctly spliced or unspliced lbx genes. B-G: Expression of myod in control (ctrl) embryos (B, C), lbx2-MO injected embryos (D, E). lbx1b-MO injected embryos (F, G). The white arrowhead indicates the sternohyoideus primordium (B) and the white arrows indicate fin muscle precursors. myod expression in fin bud is suppressed by lbx2-MO or lbx1b-MO (B: 100%, n = 36; D: 12%, n = 56; F: 16%, n = 24). H-M: Expression of fgf8 in ectodermal cells of the fin bud. Control embryos (H, I, 100%, n = 12), lbx2-MO injected embryos (J-K, 100%, n = 12), lbx1b-MO injected embryos (L-M, 100%, n = 15). (B, D, F, H, J, L) Dorsal views, rostral towards the left, (C, E, G. I, K, M) lateral views, rostral toward the left, dorsal toward the top. Scale bar: (B-M) 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
fgf8a WT control Long-pec ectoderm ISH
WT + MO1-lbx1a standard conditions Long-pec ectoderm ISH
WT + MO2-lbx2 standard conditions Long-pec ectoderm ISH
myod1 WT control Long-pec hyoid muscle ISH
Long-pec Not Detected pectoral fin musculature ISH
Long-pec Not Detected sternohyoid ISH
WT + MO1-lbx1a standard conditions Long-pec Not Detected pectoral fin musculature ISH
Long-pec Not Detected sternohyoid ISH
WT + MO2-lbx2 standard conditions Long-pec Not Detected hyoid muscle ISH
Long-pec Not Detected pectoral fin musculature ISH
Long-pec Not Detected sternohyoid ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.