ZFIN ID: ZDB-FIG-090320-46
Ochi et al., 2009 - Lbx2 regulates formation of myofibrils. BMC Developmental Biology   9:13 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody: Ab-EB165
Fish: WT, WT + MO2-lbx2
Knockdown Reagent: MO2-lbx2
Anatomical Term: fast muscle cell
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish: WT + MO2-lbx2
Knockdown Reagent: MO2-lbx2
Observed In: fast muscle cell, fast muscle cell myofibril
Stage Range: Prim-5 to Long-pec

Fig. 5 Fast muscle fibers are malformed in the absence of Lbx2. A-C: Control embryos. D-F: lbx2-MO injected embryos. G-I: lbx2 mRNA injected embryos. (A, B, D, E, G, H, J) Embryos labeled with the fast muscle marker, EB165 (red). (B, E, H) Higher magnification views. Rostral caudal extension of fast fibers is severely affected (B, E, arrow). J: Statistical analyses of mean filament rostral-caudal length (B, E, arrows) or dorsal ventral thickness (B, E, arrowhead). Analysis by ANOVA demonstrates significant differences in length (P < 0.01). (C, F, I) Embryos were mosaically labeled with membrane localized GFP (mGFP) by injection of DNA and labeled for nuclei (red, propidium iodide). mGFP expressing cells in the fast muscle domain at the level of somite 10 were randomly selected and the number of their nuclei counted. K: Fast muscle cells are multinucleate by 48 hpf in both control and lbx2 mRNA injected embryos, whereas unfused fast muscle cells are observed in lbx2-MO injected embryo (F, arrow). Analysis demonstrates significant differences among the three groups at 48 hpf (Kruskal-Wallis test, p = 0.0121). No apparent differences can be detected between controls and embryos injected with lbx2-MO at 24 hpf (Kruskal-Wallis test, p = 0.1509). (A-I) Lateral views, rostral toward the left, dorsal toward the top. Scale bars: (A, B, D, E, G, H) 50 μm, (C, F, I) 20 μm.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-EB165 IHC WT control Prim-5 - Long-pec fast muscle cell
IHC WT + MO2-lbx2 standard conditions Prim-5 - Long-pec fast muscle cell
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-lbx2 standard conditions Prim-5 - Long-pec fast muscle cell multinucleate, normal
Prim-5 - Long-pec fast muscle cell unfused from fast muscle cell, abnormal
Prim-5 - Long-pec fast muscle cell myofibril decreased length, abnormal
Prim-5 - Long-pec fast muscle cell myofibril thickness, normal
Acknowledgments:
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.