ZFIN ID: ZDB-FIG-090320-45
Ochi et al., 2009 - Lbx2 regulates formation of myofibrils. BMC Developmental Biology   9:13 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene: prox1a
Antibodies: Ab-F59, Ab1-eng, Ab5-prox1
Fish: WT, WT + MO2-lbx2
Knockdown Reagent: MO2-lbx2
Anatomical Terms: muscle pioneer, slow muscle cell
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish: WT + MO2-lbx2
Knockdown Reagent: MO2-lbx2
Observed In: muscle pioneer, muscle pioneer myofibril, slow muscle cell, slow muscle cell myofibril
Stage Range: Prim-5 to Long-pec

Fig. 4 Knockdown of Lbx2 activity results in malformation of slow muscle fibers. A-D: Control embryos. E-H: lbx2-MO injected embryos. I-L: lbx2 mRNA injected embryos. (A-B, E-F, I-J) Embryos labeled with the slow muscle marker, F59 (red). (B, F, J) Higher magnification. Rostral-caudal extension of slow fibers is severely affected by lbx2 knockdown (B, F, arrows). M: Statistical analyses of mean filament rostral-caudal length (arrows) and dorsoventral thickness (B, F, arrowheads). Analysis by ANOVA demonstrates significant differences in length (P < 0.01) and thickness (P < 0.05). (C, G, K, N) Embryos labeled with Prox1 (green, nuclear slow muscle and muscle pioneer marker), and 4D9 (red, Eng, muscle pioneer marker,). Green indicates slow muscle cells and yellow shows muscle pioneers (MP). (ctrl: n = 10, lbx2-MO: n = 6, lbx2-mRNA: n = 4). No apparent differences can be detected between controls and embryos injected with lbx2-MO. N: Analysis by ANOVA demonstrates no significant differences. The data represent the average ± s.e.m. (D, H, L) Transverse sections of a 24 hpf embryo labeled with the slow muscle marker, F59 (green) and Hoechst to mark nuclei. Slow muscle cells migrate properly to the superficial layer. (A-C, E-G, I-K) Lateral views, rostral toward the left, dorsal toward the top; (D, H, L) dorsal toward the top. Scale bar: (A, C-E, G-I, K-L) 50 μm; (B, F, J) 25 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
prox1a Ab5-prox1 WT control Prim-5 muscle pioneer IHC
Ab5-prox1 Prim-5 slow muscle cell IHC
Ab5-prox1 Prim-5 to Long-pec muscle pioneer IHC
Ab5-prox1 Prim-5 to Long-pec slow muscle cell IHC
Ab5-prox1 WT + MO2-lbx2 standard conditions Prim-5 muscle pioneer IHC
Ab5-prox1 Prim-5 slow muscle cell IHC
Ab5-prox1 Prim-5 to Long-pec muscle pioneer IHC
Ab5-prox1 Prim-5 to Long-pec slow muscle cell IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-F59 IHC WT control Prim-5 - Long-pec slow muscle cell
IHC WT + MO2-lbx2 standard conditions Prim-5 - Long-pec slow muscle cell
Ab1-eng IHC WT control Prim-5 - Long-pec muscle pioneer
IHC WT + MO2-lbx2 standard conditions Prim-5 - Long-pec muscle pioneer
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-lbx2 standard conditions Prim-5 - Long-pec muscle pioneer normal amount, normal
Prim-5 - Long-pec muscle pioneer myofibril decreased length, abnormal
Prim-5 - Long-pec muscle pioneer myofibril decreased thickness, abnormal
Prim-5 - Long-pec slow muscle cell normal amount, normal
Prim-5 - Long-pec slow muscle cell myofibril decreased length, abnormal
Prim-5 - Long-pec slow muscle cell myofibril decreased thickness, abnormal
Acknowledgments:
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.