ZFIN ID: ZDB-FIG-090320-42
Ochi et al., 2009 - Lbx2 regulates formation of myofibrils. BMC Developmental Biology   9:13 Full text @ BMC Dev. Biol.
Genes: lbx1a, lbx2, ta
Antibody: Ab-F59
Fish: WT
Anatomical Terms: adaxial cell, epaxial myotome region, fast muscle cell, head muscle, hindbrain (all 14) expand
Stage Range: 75%-epiboly to Long-pec

Fig. 1 Muscle precursors express lbx1b and lbx2. A-L: Muscle precursors transiently express lbx2. A-G, L: Expression of lbx2 at 70%-epiboly (A), 90%-epiboly (B), bud (C), segmentation (D), 24 hpf (E, L), 48 hfp (F, G). Whole-mounts. H-K: lbx2 (blue) and ntl (red) expression at 70%-epiboly (H), 90%-epiboly (I), bud (J), and segmentation (K). Flat-mounts. L: Transverse section, 24 hpf embryo, F59 (green) and lbx2 mRNA (blue). (A, H) lbx2 mRNA appears at 70%-epiboly adjacent to ntl expressing cells in blastoderm margin. lbx2 expression in paraxial mesoderm by end of gastrulation (B-C, I,). lbx2 expression later restricted to adaxial cells (J-K, brackets). (L) Subset of fast muscle cells expresses lbx2 in epaxial (black arrow) and hypaxial (black arrowhead) domains. F59 and lbx2 labeling shows differentiated slow muscle cells lose lbx2 expression (L, white arrowhead). (F) lbx2 expression in trunk disappears by 48 hpf (F, bracket). lbx2 expression in fin primordia (F-G, black arrowheads), hindbrain (F-G, white arrowhead), and hyoid (F, white arrow). M: Diagram of zebrafish muscle. Adaxial cells (K, bracket) migrate superficially and differentiate into slow muscle fibers (green). Then, fast (magenta) and medial fast fibers (red) differentiate. N-R: 5-somite (N), 18-somite (O), 24 hpf (P-Q) and 48 hpf (R). (Q) Higher magnification of P. lbx1b mRNA appears at 5-somite stage in neural tube (N), along rostral-caudal axis (O, arrow), then lateral to somites (P-Q), and later in fin (R). (A-D, N-O) Dorsal views, rostral towards the top; (E-F, P) lateral views, rostral toward the left, dorsal toward the top; (H-K) rostral toward the top; (L) dorsal toward the top; (G, R) dorsal views, rostral toward the left. Scale bars: (A-D, N-O, Q) 200 μm, (E-G, P, R) 100 μm, (H-L) 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
lbx1a WT standard conditions 5-9 somites neural rod ISH
14-19 somites pectoral fin field ISH
Prim-5 pectoral fin bud ISH
Prim-5 pectoral fin field ISH
Long-pec pectoral fin ISH
lbx2 WT standard conditions 75%-epiboly head muscle ISH
90%-epiboly head muscle ISH
Bud adaxial cell ISH
Bud paraxial mesoderm ISH
10-13 somites adaxial cell ISH
Prim-5 epaxial myotome region ISH
Prim-5 fast muscle cell ISH
Prim-5 hypaxial myotome region ISH
Prim-5 Not Detected slow muscle cell ISH
Long-pec hindbrain ISH
Long-pec hyoid muscle ISH
Long-pec pectoral fin ISH
ta WT standard conditions 75%-epiboly margin ISH
90%-epiboly margin ISH
Bud margin ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-F59 IHC WT control Prim-5 slow muscle cell
Phenotype Details No data available
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.