Fig. 1

Broad expression of transcription factor and transcriptional regulator genes in wholemount zebrafish embryos at 24 h. (A?V) Lateral views of wholemount WT zebrafish embryos at 24 h. A minimum of five embryos were analyzed in detail per gene to determine the representative expression pattern (see Experimental Procedures for further details). (W) Schematic of a lateral view of a 24 h wholemount zebrafish embryo. F, forebrain, M, midbrain, H, hindbrain, V, mid- and hindbrain ventricles, SC, spinal cord, N, notochord, B, blood (black dotted line indicates boundary between dorsal aorta and cardinal vein), Y, yolk and the eye and lens are indicated with concentric blue dotted circles. In all panels, rostral is left and dorsal is up. Transcriptional regulator gene (A) aurkb, and transcription factor genes (B) foxb1a, (C) foxb1b, (D) her8a, (E) homeza, (F) ivns1abpb, (G) mybl2b, (H) myt1a, (I) nr2f1b, (J) onecut1, (K) sall1a, (L) sall3a, (M) sall3b, (N) sall4, (O) sox2 (P), sox19b, (Q) sp8b, (R) tsc22d1, (S) wdhd1, (T) zfhx3b, (U) znf804a, and (V) znf1032 are all broadly expressed throughout the rostro-caudal and dorso-ventral spinal cord and all 22 of these genes are also variably expressed in the brain. (A) aurkb, (F) ivns1abpb, (G) mybl2b, (N) sall4, (S) wdhd1, (U) znf804a, and (V) znf1032 are also variably expressed in the blood beneath the notochord. (N) sall4 in situ hybridization experiments were performed with the molecular crowding reagent Dextran Sulfate (see Experimental Procedures for rationale). All other in situ hybridization experiments in this figure were performed without this reagent. Scale bar: 200 ?m.