A subset of transcription factor and transcriptional regulator genes lose expression in the spinal cord of zebrafish mib1ta52b mutant embryos at 24 h. (A?Y) Lateral views of (A, C, F, H, K, M, P, R, U, W) head, (B, D, G, I, L, N, Q, S, V, X) spinal cord, and (E, J, O, T, Y) tail in (A, B, F, G, K, L, P, Q, U, V) sibling and (C?E, H?J, M?O, R?T, W?Y) mib1ta52b mutant embryos at 24 h. Rostral, left. Dorsal, up. A minimum of five embryos were analyzed per gene for each genotype to determine representative expression patterns (see Experimental Procedures). None of the in situ hybridization experiments in this figure were performed with the molecular crowding reagent Dextran Sulfate. Scale bar: (A, C, F, H, K, M, P, R, U, W) 50 ?m, (B, D, G, I, L, N, Q, S, V, X) 20 ?m, and (E, J, O, T, Y) 25 ?m.
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