Fig. 2

Expression of pan-mesodermal candidate genes.

The majority of the identified genes show early dorsal expression in wild type embryos and lack thereof in ich controls. This is followed by a second wave of circumferential, mesodermal expression, which could be detected in both genetic backgrounds. An interesting exception is flh, which shows a somewhat counterintuitive expression dynamics: a very strong circumferential expression (complemented by strong dorsal signal in wild type embryos) can be detected as early as late sphere stage (Ei, Eii), but this expression becomes significantly weaker by 30% epiboly, except in the dorsal side of wild type embryos (Eiii, Eiv). Sphere stage embryos are shown from a lateral view, whereas ~30% epiboly stage embryos are presented from an animal view (dorsal to the right in both cases).