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ZFIN ID: ZDB-FIG-130930-3
Fodor et al., 2013 - Full transcriptome analysis of early dorsoventral patterning in zebrafish. PLoS One   8(7):e70053 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 30%-epiboly to 1-4 somites

Fig. 4

Expression patterns of newly characterized genes.

(A) ier2 expression starts early in the future dorsal side, persists until early mid-gastrulation, then fades away, and becomes detectable only during somitogenesis in a stripe in the hindbrain area, and in the tailbud. (B) rnd1l expression is weakly upregulated in all the blastomeres after MBT, and it is expressed at higher levels in a few dorsal precursors. Later the expression becomes restricted in the mesoderm (both axial and non-axial), where it is progressively downregulated. During somitogenesis two prominent neural expression domains appear: in the eyefield and in the hindbrain. (C) ppp1r3ca expression starts at the dorsal edge of the embryo, and later it is upregulated in a narrow stripe around the germring. Expression weakens during late gastrula stages, but it will be upregulated during somitogenesis in the anterior forebrain and in the posterior tailbud regions. (D) LOC100334443 expression is strongly upregulated after the onset of zygotic transcription. During gastrulation it becomes restricted to the ectoderm, where it is gradually downregulated, except in the dorsal forerunner cells. During somitogenesis expression is also prominent in the neurectoderm, especially in the hindbrain. All embryos are shown from lateral view, dorsal to the right.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ier2b EKW standard conditions 30%-epiboly whole organism dorsal region ISH
Sphere whole organism dorsal region ISH
Shield whole organism dorsal region ISH
1-4 somites hindbrain neural keel ISH
1-4 somites tail bud ISH
ppp1r3ca EKW standard conditions 30%-epiboly whole organism dorsal region ISH
Sphere whole organism dorsal region ISH
Shield germ ring ISH
1-4 somites forebrain neural keel ISH
1-4 somites tail bud ISH
rnd1b EKW standard conditions Sphere blastomere ISH
30%-epiboly presumptive mesoderm ISH
Shield germ ring ISH
1-4 somites hindbrain neural keel ISH
1-4 somites optic primordium ISH
sb:cb81 EKW standard conditions Sphere whole organism ISH
30%-epiboly whole organism ISH
Shield epiblast ISH
1-4 somites hindbrain neural keel ISH
1-4 somites neuroectoderm ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One