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ZFIN ID: ZDB-FIG-111014-26
Lu et al., 2011 - Identification and mechanism of regulation of the zebrafish dorsal determinant. Proceedings of the National Academy of Sciences of the United States of America   108(38):15876-80 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 2-cell to Adult

Fig. 1 The secreted Wnt ligand Wnt8a is a strong candidate for the dorsal determinant in zebrafish. Expression of chordin at the sphere stage is shown in (A) wild type (WT), (B) an embryo injected with 500 pg of Frzb mRNA, and (C) an embryo injected with 100 pg of mRNA coding for a dominant-negative Tcf-3 (DN-Tcf3) (34). Embryos are in animal pole view with dorsal to the Right. (D) Expression of the 23 Wnt genes present in the zebrafish genome analyzed by RT-PCR at 1- to 2-cell stage (0.5 h), 1,000-cell stage (3 h), sphere stage (4 h), 40% epiboly (5 h), 60% epiboly (7 h), and at 24 hpf. Arrowheads indicate Wnt genes for which mRNAs are maternally deposited in the egg. (EJ) Whole mount in situ hybridization reveals that Wnt8a mRNAs accumulate in the Balbiani body (bb) in stage I oocytes (E) and are localized in a vegetal position in stage II (F) and stage III oocytes (G). Transcripts of Wnt8a are also observed in cleavage-stage embryos both in blastomeres and in the yolk cortical cytoplasm in vegetal position at (H) the 2-cell stage and (I) 16-cell stage. (J) Treatment with nocodazole, which depolymerizes microtubules, prevents transport of Wnt8a mRNA. Embryos are in lateral view with dorsal to the Right. Arrowheads in HJ indicate the limits of Wnt8a mRNA localization in the cortical cytoplasm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
chrd WT standard conditions Sphere whole organism ISH
wnt1 WT standard conditions 1-cell whole organism RTPCR
1k-cell whole organism RTPCR
Sphere whole organism RTPCR
30%-epiboly whole organism RTPCR
Shield Not Detected whole organism RTPCR
Prim-5 whole organism RTPCR
wnt2 WT standard conditions 1-cell whole organism RTPCR
1k-cell whole organism RTPCR
Sphere whole organism RTPCR
30%-epiboly whole organism RTPCR
Shield whole organism RTPCR
Prim-5 whole organism RTPCR
wnt2ba WT standard conditions 1-cell Not Detected whole organism RTPCR
1k-cell Not Detected whole organism RTPCR
30%-epiboly Not Detected whole organism RTPCR
Sphere Not Detected whole organism RTPCR
Shield whole organism RTPCR
Prim-5 whole organism RTPCR
wnt2bb WT standard conditions 1-cell whole organism RTPCR
1k-cell whole organism RTPCR
Sphere whole organism RTPCR
30%-epiboly whole organism RTPCR
Shield whole organism RTPCR
Prim-5 whole organism RTPCR
wnt3 WT standard conditions 1-cell Not Detected whole organism RTPCR
1k-cell whole organism RTPCR
Sphere whole organism RTPCR
30%-epiboly Not Detected whole organism RTPCR
Shield whole organism RTPCR
Prim-5 whole organism RTPCR
wnt3a WT standard conditions 1-cell Not Detected whole organism RTPCR
1k-cell Not Detected whole organism RTPCR
Sphere whole organism RTPCR
30%-epiboly whole organism RTPCR
Shield whole organism RTPCR
Prim-5 whole organism RTPCR
wnt4 WT standard conditions 1-cell whole organism RTPCR
1k-cell whole organism RTPCR
30%-epiboly whole organism RTPCR
Sphere whole organism RTPCR
Shield whole organism RTPCR
Prim-5 whole organism RTPCR
wnt4b WT standard conditions 1-cell whole organism RTPCR
1k-cell whole organism RTPCR
Sphere whole organism RTPCR
30%-epiboly whole organism RTPCR
Shield Not Detected whole organism RTPCR
Prim-5 whole organism RTPCR
wnt5a WT standard conditions 1-cell whole organism RTPCR
1k-cell whole organism RTPCR
30%-epiboly whole organism RTPCR
Sphere whole organism RTPCR
Shield whole organism RTPCR
Prim-5 whole organism RTPCR
wnt5b WT standard conditions 1-cell Not Detected whole organism RTPCR
1k-cell whole organism RTPCR
30%-epiboly whole organism RTPCR
Sphere whole organism RTPCR
Shield whole organism RTPCR
Prim-5 whole organism RTPCR
wnt6b WT standard conditions 1-cell whole organism RTPCR
1k-cell Not Detected whole organism RTPCR
Sphere Not Detected whole organism RTPCR
30%-epiboly Not Detected whole organism RTPCR
Shield Not Detected whole organism RTPCR
Prim-5 whole organism RTPCR
wnt7aa WT standard conditions 1-cell whole organism RTPCR
1k-cell whole organism RTPCR
Sphere whole organism RTPCR
30%-epiboly whole organism RTPCR
Shield whole organism RTPCR
Prim-5 whole organism RTPCR
wnt7ba WT standard conditions 1-cell Not Detected whole organism RTPCR
1k-cell whole organism RTPCR
30%-epiboly whole organism RTPCR
Sphere whole organism RTPCR
Shield whole organism RTPCR
Prim-5 whole organism RTPCR
wnt7bb WT standard conditions 1-cell Not Detected whole organism RTPCR
1k-cell Not Detected whole organism RTPCR
Sphere whole organism RTPCR
30%-epiboly whole organism RTPCR
Shield whole organism RTPCR
Prim-5 whole organism RTPCR
wnt8a WT standard conditions 2-cell whole organism ISH
1-cell whole organism RTPCR
16-cell whole organism ISH
2-cell yolk ISH
16-cell yolk ISH
1k-cell whole organism RTPCR
30%-epiboly whole organism RTPCR
Sphere whole organism RTPCR
Shield whole organism RTPCR
Prim-5 whole organism RTPCR
Adult oocyte stage I mitochondrial cloud ISH
Adult oocyte stage II ISH
Adult oocyte stage III ISH
wnt8b WT standard conditions 1-cell whole organism RTPCR
1k-cell Not Detected whole organism RTPCR
Sphere whole organism RTPCR
30%-epiboly whole organism RTPCR
Shield whole organism RTPCR
Prim-5 whole organism RTPCR
wnt9a WT standard conditions 1-cell whole organism RTPCR
1k-cell whole organism RTPCR
30%-epiboly whole organism RTPCR
Sphere whole organism RTPCR
Shield whole organism RTPCR
Prim-5 whole organism RTPCR
wnt9b WT standard conditions 1-cell whole organism RTPCR
1k-cell whole organism RTPCR
30%-epiboly whole organism RTPCR
Sphere whole organism RTPCR
Shield whole organism RTPCR
Prim-5 whole organism RTPCR
wnt10a WT standard conditions 1-cell whole organism RTPCR
1k-cell whole organism RTPCR
30%-epiboly whole organism RTPCR
Sphere whole organism RTPCR
Shield whole organism RTPCR
Prim-5 whole organism RTPCR
wnt10b WT standard conditions 1-cell whole organism RTPCR
1k-cell whole organism RTPCR
Sphere whole organism RTPCR
30%-epiboly whole organism RTPCR
Shield whole organism RTPCR
Prim-5 whole organism RTPCR
wnt11 WT standard conditions 1-cell whole organism RTPCR
1k-cell whole organism RTPCR
Sphere whole organism RTPCR
30%-epiboly whole organism RTPCR
Shield whole organism RTPCR
Prim-5 whole organism RTPCR
wnt11f2 WT standard conditions 1-cell whole organism RTPCR
1k-cell whole organism RTPCR
30%-epiboly whole organism RTPCR
Sphere whole organism RTPCR
Shield whole organism RTPCR
Prim-5 whole organism RTPCR
wnt16 WT standard conditions 1-cell whole organism RTPCR
1k-cell whole organism RTPCR
Sphere whole organism RTPCR
30%-epiboly whole organism RTPCR
Shield whole organism RTPCR
Prim-5 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA