FIGURE

Fig. S3

ID

ZDB-FIG-150108-10

Publication

Lu et al., 2011 - Identification and mechanism of regulation of the zebrafish dorsal determinant

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Fig. S3

Assay of activation of the maternal β-catenin signaling pathway by canonical and noncanonical Wnt ligands. mRNA coding for canonical or noncanonical Wnts was injected together with GFP mRNA into one animal pole blastomere at the 64-cell stage. Embryos displaying a fluorescent clone at the animal pole were fixed at the sphere stage and the activation of the maternal β-catenin visualized by looking, by whole mount in situ hybridization, at the induction of ectopic expression of dharma at the animal pole. Wnt3a (B) and Wnt8a (G) are the only Wnts able to induce ectopic dharma expression. This is observed with as little as 10 pg of mRNA injected. All other Wnt ligands, canonical Wnts [Wnt2 (A), Wnt9b (H), and Wnt10b (I)], noncanonical Wnts [Wnt4a (C), Wnt4b (D), Wnt5a (E), Wnt5b (F), Wnt11 (J), and Wnt11r (K)], or the combination Wnt11–Wnt5a (L), which has been described to act as dorsal determinant in amphibian, are unable to efficiently activate the maternal β-catenin signaling pathway and fail to induce ectopic expression of dharma at the animal pole, even for 200 pg of mRNA injected. For each Wnt, assayed embryos are presented in both animal pole view (Left) and lateral view (Right). The amount of mRNA injected is indicated in the Lower Right corner.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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