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ZFIN ID: ZDB-FIG-150504-22
Kantarci et al., 2015 - Tfap2a Promotes Specification and Maturation of Neurons in the Inner Ear through Modulation of Bmp, Fgf and Notch Signaling. PLoS Genetics   11:e1005037 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Fig. 6 Tfap2a regulates the level of Fgf and Notch Signaling in the otic vesicle.

(A-V) Whole-mount images (dorsal up, anterior left) showing dorsolateral views of the otic vesicle (outlined). (A-R) Expression of the indicated genes in wild-type embryos, hs:tfap2a embryos and tfap2a-/- mutants (A-I) or tfap2a morphants (J-R) at 26 hpf (A-L, P-R) and 28 (M-O) hpf. (S, T) Cross-sections (dorsal up, medial left) passing through the utricular macula show spry4 expression at 28 hpf in a control embryo and tfap2a-/- mutant. (U-X) Whole-mounts showing expression of etv5b at 26 hpf. Activation of hs:tfap2a diminishes etv5b expression (U, V), activation of hs:fgf8 leads to global upregulation of etv5b (W), and co-activation of hs:fgf8 and hs:tfap2a restores etv5b to near normal (X). (Y) Cross-sections (dorsal up, medial left) passing just posterior to the utricular macula showing ngn1 at 24 hpf following a 35°C heat shock at 23 hpf. Reduction in the ngn1 domain caused by knockdown of tfap2a is rescued by weak activation of hs:dnfgfr1. (Z) Mean and standard deviation of the total number of ngn1 positive cells in the otic epithelium at 24 hpf for the genotypes and knockdowns indicated in the color key (counted from serial sections, n = 3–6 ears per time point). Asterisks (*) indicate statistically significant differences between the groups indicated in brackets.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dla AB heat shock Prim-5 otic vesicle ISH
tfap2am819/m819 standard conditions Prim-5 otic vesicle ISH
x24Tg heat shock Prim-5 otic vesicle ISH
dlb AB heat shock Prim-5 otic vesicle ISH
tfap2am819/m819 standard conditions Prim-5 otic vesicle ISH
x24Tg heat shock Prim-5 otic vesicle ISH
etv5b AB heat shock Prim-5 otic vesicle ISH
x17Tg heat shock Prim-5 otic vesicle ISH
x17Tg; x24Tg heat shock Prim-5 otic vesicle ISH
x24Tg heat shock Prim-5 otic vesicle ISH
fgf3 AB heat shock Prim-5 otic vesicle ISH
AB + MO4-tfap2a standard conditions Prim-5 otic vesicle ISH
x24Tg heat shock Prim-5 otic vesicle ISH
fgf8a AB heat shock Prim-5 otic vesicle ISH
AB + MO4-tfap2a standard conditions Prim-5 otic vesicle ISH
x24Tg heat shock Prim-5 otic vesicle ISH
fgf10a AB heat shock Prim-5 otic vesicle ISH
AB + MO4-tfap2a standard conditions Prim-5 otic vesicle ISH
x24Tg heat shock Prim-5 otic vesicle ISH
her4.1 AB heat shock Prim-5 otic vesicle ISH
tfap2am819/m819 standard conditions Prim-5 otic vesicle ISH
x24Tg heat shock Prim-5 otic vesicle ISH
neurog1 AB heat shock Prim-5 otic vesicle ISH
AB + MO4-tfap2a heat shock Prim-5 otic vesicle ISH
pd1Tg heat shock Prim-5 otic vesicle ISH
pd1Tg + MO4-tfap2a heat shock Prim-5 otic vesicle ISH
spry4 AB control Prim-5 otic vesicle ISH
tfap2am819/m819 control Prim-5 otic vesicle ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB heat shock Prim-5 otic vesicle neuroblast fate specification occurrence, normal
AB + MO4-tfap2a heat shock Prim-5 otic vesicle neuroblast fate specification decreased occurrence, abnormal
pd1Tg heat shock Prim-5 otic vesicle neuroblast fate specification occurrence, normal
pd1Tg + MO4-tfap2a heat shock Prim-5 otic vesicle neuroblast fate specification occurrence, normal
tfap2am819/m819 standard conditions Prim-5 otic vesicle fibroblast growth factor receptor signaling pathway increased process quality, abnormal
Prim-5 otic vesicle Notch signaling pathway increased process quality, abnormal
x24Tg heat shock Prim-5 otic vesicle fibroblast growth factor receptor signaling pathway decreased process quality, abnormal
Prim-5 otic vesicle Notch signaling pathway decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.