ZFIN ID: ZDB-FIG-150504-24
Kantarci et al., 2015 - Tfap2a Promotes Specification and Maturation of Neurons in the Inner Ear through Modulation of Bmp, Fgf and Notch Signaling. PLoS Genetics   11:e1005037 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Conditions:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage Range: High-pec to Protruding-mouth

Fig. 8 Tfap2a regulates development of TA cells independent of earlier stages.

(A-L) Cross-sections (medial left, dorsal up) at the level of utricular macula. The otic vesicle and regions occupied by mature SAG neurons and TA cells are outlined in each image. Co-staining for Isl1 (red) and neurod (blue) at 50 hpf (A-D) and 72 hpf (E-H) under conditions indicated across the top of the figure. All specimens were treated with 0.3% DMSO and heat-shocked (39°C) at 40 hpf. (I-L) BrdU staining in embryos exposed to BrdU for 5 hours, from 40 to 45 hpf. The region containing mature SAG neurons is indicated. (M-O) Mean and standard deviation for the total number of neurod+ TA cells (M), total Isl1+ mature neurons (N) and total BrdU+ TA cells (O) for the indicated genotypes and treatments (counted from serial sections, n = 3–6 ears per time point). Differences between control and experimental specimens were significant at all time points, except that hs:fgf8+hs:gal4/UAS-NICD embryos were not significantly different from control embryos at 72 hpf in (N). (P) Percentage of neurod+ TA cells that are also BrdU positive (counted from serial sections, n = 3–6 ears per time point). Only hs:fgf8+hs:gal4/UAS-NICD embryos were significantly different from the control in (P).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
neurod1 AB heat shock Long-pec statoacoustic (VIII) ganglion neuroblast (sensu Vertebrata) ISH
Protruding-mouth statoacoustic (VIII) ganglion neuroblast (sensu Vertebrata) ISH
kca3Tg; kca4Tg; x17Tg heat shock Long-pec statoacoustic (VIII) ganglion neuroblast (sensu Vertebrata) ISH
Protruding-mouth statoacoustic (VIII) ganglion neuroblast (sensu Vertebrata) ISH
pd1Tg heat shock, chemical treatment: LY-411575 Long-pec statoacoustic (VIII) ganglion neuroblast (sensu Vertebrata) ISH
Protruding-mouth statoacoustic (VIII) ganglion neuroblast (sensu Vertebrata) ISH
x24Tg heat shock Long-pec statoacoustic (VIII) ganglion neuroblast (sensu Vertebrata) ISH
Protruding-mouth statoacoustic (VIII) ganglion neuroblast (sensu Vertebrata) ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab2-isl IHC AB heat shock Long-pec statoacoustic (VIII) ganglion neuron
IHC Protruding-mouth statoacoustic (VIII) ganglion neuron
IHC kca3Tg; kca4Tg; x17Tg heat shock Long-pec statoacoustic (VIII) ganglion neuron
IHC Protruding-mouth statoacoustic (VIII) ganglion neuron
IHC pd1Tg heat shock, chemical treatment: LY-411575 Long-pec statoacoustic (VIII) ganglion neuron
IHC Protruding-mouth statoacoustic (VIII) ganglion neuron
IHC x24Tg heat shock Long-pec statoacoustic (VIII) ganglion neuron
IHC Protruding-mouth statoacoustic (VIII) ganglion neuron
Phenotype Details
Fish Conditions Stage Phenotype
kca3Tg; kca4Tg; x17Tg heat shock High-pec statoacoustic (VIII) ganglion neuroblast proliferation increased occurrence, abnormal
Long-pec statoacoustic (VIII) ganglion has extra parts of type statoacoustic (VIII) ganglion neuroblast (sensu Vertebrata), abnormal
Long-pec statoacoustic (VIII) ganglion has fewer parts of type statoacoustic (VIII) ganglion neuron, abnormal
Long-pec statoacoustic (VIII) ganglion neuron differentiation delayed, abnormal
Protruding-mouth statoacoustic (VIII) ganglion has extra parts of type statoacoustic (VIII) ganglion neuroblast (sensu Vertebrata), abnormal
Protruding-mouth statoacoustic (VIII) ganglion has fewer parts of type statoacoustic (VIII) ganglion neuron, abnormal
Protruding-mouth statoacoustic (VIII) ganglion neuron differentiation delayed, abnormal
pd1Tg heat shock, chemical treatment: LY-411575 High-pec statoacoustic (VIII) ganglion neuroblast proliferation occurrence, normal
Long-pec statoacoustic (VIII) ganglion has extra parts of type statoacoustic (VIII) ganglion neuron, abnormal
Long-pec statoacoustic (VIII) ganglion has fewer parts of type statoacoustic (VIII) ganglion neuroblast (sensu Vertebrata), abnormal
Protruding-mouth statoacoustic (VIII) ganglion has extra parts of type statoacoustic (VIII) ganglion neuron, abnormal
Protruding-mouth statoacoustic (VIII) ganglion has fewer parts of type statoacoustic (VIII) ganglion neuroblast (sensu Vertebrata), abnormal
x24Tg heat shock High-pec statoacoustic (VIII) ganglion neuroblast proliferation occurrence, normal
Long-pec statoacoustic (VIII) ganglion has extra parts of type statoacoustic (VIII) ganglion neuron, abnormal
Long-pec statoacoustic (VIII) ganglion has fewer parts of type statoacoustic (VIII) ganglion neuroblast (sensu Vertebrata), abnormal
Protruding-mouth statoacoustic (VIII) ganglion has extra parts of type statoacoustic (VIII) ganglion neuron, abnormal
Protruding-mouth statoacoustic (VIII) ganglion has fewer parts of type statoacoustic (VIII) ganglion neuroblast (sensu Vertebrata), abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.