ZFIN ID: ZDB-FIG-150504-24
Kantarci et al., 2015 - Tfap2a Promotes Specification and Maturation of Neurons in the Inner Ear through Modulation of Bmp, Fgf and Notch Signaling. PLoS Genetics   11:e1005037 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Conditions:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage Range: High-pec to Protruding-mouth

Fig. 8 Tfap2a regulates development of TA cells independent of earlier stages.

(A-L) Cross-sections (medial left, dorsal up) at the level of utricular macula. The otic vesicle and regions occupied by mature SAG neurons and TA cells are outlined in each image. Co-staining for Isl1 (red) and neurod (blue) at 50 hpf (A-D) and 72 hpf (E-H) under conditions indicated across the top of the figure. All specimens were treated with 0.3% DMSO and heat-shocked (39°C) at 40 hpf. (I-L) BrdU staining in embryos exposed to BrdU for 5 hours, from 40 to 45 hpf. The region containing mature SAG neurons is indicated. (M-O) Mean and standard deviation for the total number of neurod+ TA cells (M), total Isl1+ mature neurons (N) and total BrdU+ TA cells (O) for the indicated genotypes and treatments (counted from serial sections, n = 3–6 ears per time point). Differences between control and experimental specimens were significant at all time points, except that hs:fgf8+hs:gal4/UAS-NICD embryos were not significantly different from control embryos at 72 hpf in (N). (P) Percentage of neurod+ TA cells that are also BrdU positive (counted from serial sections, n = 3–6 ears per time point). Only hs:fgf8+hs:gal4/UAS-NICD embryos were significantly different from the control in (P).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
neurod1 AB heat shock Long-pec statoacoustic (VIII) ganglion neuroblast (sensu Vertebrata) ISH
Protruding-mouth statoacoustic (VIII) ganglion neuroblast (sensu Vertebrata) ISH
kca3Tg; kca4Tg; x17Tg heat shock Long-pec statoacoustic (VIII) ganglion neuroblast (sensu Vertebrata) ISH
Protruding-mouth statoacoustic (VIII) ganglion neuroblast (sensu Vertebrata) ISH
pd1Tg heat shock, chemical treatment: LY-411575 Long-pec statoacoustic (VIII) ganglion neuroblast (sensu Vertebrata) ISH
Protruding-mouth statoacoustic (VIII) ganglion neuroblast (sensu Vertebrata) ISH
x24Tg heat shock Long-pec statoacoustic (VIII) ganglion neuroblast (sensu Vertebrata) ISH
Protruding-mouth statoacoustic (VIII) ganglion neuroblast (sensu Vertebrata) ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab2-isl IHC AB heat shock Long-pec statoacoustic (VIII) ganglion neuron
IHC Protruding-mouth statoacoustic (VIII) ganglion neuron
IHC kca3Tg; kca4Tg; x17Tg heat shock Long-pec statoacoustic (VIII) ganglion neuron
IHC Protruding-mouth statoacoustic (VIII) ganglion neuron
IHC pd1Tg heat shock, chemical treatment: LY-411575 Long-pec statoacoustic (VIII) ganglion neuron
IHC Protruding-mouth statoacoustic (VIII) ganglion neuron
IHC x24Tg heat shock Long-pec statoacoustic (VIII) ganglion neuron
IHC Protruding-mouth statoacoustic (VIII) ganglion neuron
Phenotype Details
Fish Conditions Stage Phenotype
kca3Tg; kca4Tg; x17Tg heat shock High-pec statoacoustic (VIII) ganglion neuroblast proliferation increased occurrence, abnormal
Long-pec statoacoustic (VIII) ganglion has extra parts of type statoacoustic (VIII) ganglion neuroblast (sensu Vertebrata), abnormal
Long-pec statoacoustic (VIII) ganglion has fewer parts of type statoacoustic (VIII) ganglion neuron, abnormal
Long-pec statoacoustic (VIII) ganglion neuron differentiation delayed, abnormal
Protruding-mouth statoacoustic (VIII) ganglion has extra parts of type statoacoustic (VIII) ganglion neuroblast (sensu Vertebrata), abnormal
Protruding-mouth statoacoustic (VIII) ganglion has fewer parts of type statoacoustic (VIII) ganglion neuron, abnormal
Protruding-mouth statoacoustic (VIII) ganglion neuron differentiation delayed, abnormal
pd1Tg heat shock, chemical treatment: LY-411575 High-pec statoacoustic (VIII) ganglion neuroblast proliferation occurrence, normal
Long-pec statoacoustic (VIII) ganglion has extra parts of type statoacoustic (VIII) ganglion neuron, abnormal
Long-pec statoacoustic (VIII) ganglion has fewer parts of type statoacoustic (VIII) ganglion neuroblast (sensu Vertebrata), abnormal
Protruding-mouth statoacoustic (VIII) ganglion has extra parts of type statoacoustic (VIII) ganglion neuron, abnormal
Protruding-mouth statoacoustic (VIII) ganglion has fewer parts of type statoacoustic (VIII) ganglion neuroblast (sensu Vertebrata), abnormal
x24Tg heat shock High-pec statoacoustic (VIII) ganglion neuroblast proliferation occurrence, normal
Long-pec statoacoustic (VIII) ganglion has extra parts of type statoacoustic (VIII) ganglion neuron, abnormal
Long-pec statoacoustic (VIII) ganglion has fewer parts of type statoacoustic (VIII) ganglion neuroblast (sensu Vertebrata), abnormal
Protruding-mouth statoacoustic (VIII) ganglion has extra parts of type statoacoustic (VIII) ganglion neuron, abnormal
Protruding-mouth statoacoustic (VIII) ganglion has fewer parts of type statoacoustic (VIII) ganglion neuroblast (sensu Vertebrata), abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.