ZFIN ID: ZDB-FIG-150504-25
Kantarci et al., 2015 - Tfap2a Promotes Specification and Maturation of Neurons in the Inner Ear through Modulation of Bmp, Fgf and Notch Signaling. PLoS Genetics   11:e1005037 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Prim-5

Fig. 9 Tfap2a regulates SAG development non-autonomously.

(A-D) Whole-mounts (dorsal up, anterior left) and cross-sections (just posterior to utricular macula, medial to the left) showing both bright-field and corresponding fluorescent images of +/+ host embryos (A, B) and hs:tfap2a host embryos stained for etv5b expression (blue) and showing transplanted wild-type donor cells (red). Positions of wild-type cells that fail to express etv5b are highlighted with arrows in (C, D). (E) Summary of mosaic analysis showing the number of +/+ or hs:tfap2a host embryos examined and the number of +/+ donor cells expressing etv5b over the total number of donor cells that populated the ventral half of the otic vesicle. (F-H) Expression of etv5b at 26 hpf in a control (F), a DM-treated hs:tfap2a embryo (G) and DM-treated wild-type embryo (H). All specimens were treated with 1% DMSO and heat-shocked at 24 hpf. (I-L) Expression of bmp7a at 26 hpf in a control (I, J), tfap2a-/- mutant (K) and hs:tfap2a embryo (L). Wild-type and hs:tfap2a embryos were heat shocked at 24 hpf.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
bmp7a AB heat shock Prim-5 otic vesicle anterior region ISH
Prim-5 otic vesicle posterior region ISH
Prim-5 otic vesicle ventro-lateral region ISH
tfap2am819/m819 standard conditions Prim-5 Not Detected otic vesicle anterior region ISH
Prim-5 otic vesicle posterior region ISH
Prim-5 Not Detected otic vesicle ventro-lateral region ISH
x24Tg heat shock Prim-5 otic vesicle anterior region ISH
Prim-5 otic vesicle posterior region ISH
Prim-5 otic vesicle ventro-lateral region ISH
etv5b AB heat shock Prim-5 otic vesicle ISH
AB heat shock, chemical treatment: dorsomorphin Prim-5 otic vesicle ISH
x24Tg heat shock, chemical treatment: dorsomorphin Prim-5 otic vesicle ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB heat shock, chemical treatment: dorsomorphin Prim-5 otic vesicle fibroblast growth factor receptor signaling pathway process quality, normal
x24Tg heat shock, chemical treatment: dorsomorphin Prim-5 otic vesicle fibroblast growth factor receptor signaling pathway process quality, normal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.