FIGURE

Fig. 5

ID

ZDB-FIG-150504-21

Publication

Kantarci et al., 2015 - Tfap2a Promotes Specification and Maturation of Neurons in the Inner Ear through Modulation of Bmp, Fgf and Notch Signaling

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Effects of tfap2a knockdown and overexpression on otic vesicle patterning.

(A-L) Whole-mount images (dorsal up, anterior left) showing dorsolateral views of the otic vesicle (outlined) in control embryos, tfap2a^-/- mutants and tfap2a morphants, and hs:tfap2a embryos for the indicated genes at 26 hpf. (M) Mean and standard deviation of the total number of hair cells in utricular and saccular maculae of control and hs:tfap2a embryos and tfap2a morphants at the indicated times (n = 24 embryos each). Data were obtained by counting GFP-positive hair cells in the sensory epithelia of brn3c:Gfp transgenic embryos. Accumulation of hair cells was normal except in tfap2a morphants at 36 hpf (*), which showed a small but significant decrease relative to the control.

Expression Data

Genes:	dlx3b otx1 pax5 pou3f3b
Fish:	AB tfap2a^m819/m819 AB + MO4-tfap2a x24Tg
Condition:	heat shock
Knockdown Reagent:	MO4-tfap2a
Anatomical Terms:	otic vesicle antero-ventral region otic vesicle dorsal region otic vesicle postero-medial region otic vesicle ventro-lateral region
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tfap2a^m819/m819 AB + MO4-tfap2a x24Tg
Condition:	heat shock
Knockdown Reagent:	MO4-tfap2a
Observed In:	otic vesicle otic vesicle hair cell otic vesicle hair cell differentiation
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.