ZFIN ID: ZDB-FIG-150504-21
Kantarci et al., 2015 - Tfap2a Promotes Specification and Maturation of Neurons in the Inner Ear through Modulation of Bmp, Fgf and Notch Signaling. PLoS Genetics   11:e1005037 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 5 Effects of tfap2a knockdown and overexpression on otic vesicle patterning.

(A-L) Whole-mount images (dorsal up, anterior left) showing dorsolateral views of the otic vesicle (outlined) in control embryos, tfap2a-/- mutants and tfap2a morphants, and hs:tfap2a embryos for the indicated genes at 26 hpf. (M) Mean and standard deviation of the total number of hair cells in utricular and saccular maculae of control and hs:tfap2a embryos and tfap2a morphants at the indicated times (n = 24 embryos each). Data were obtained by counting GFP-positive hair cells in the sensory epithelia of brn3c:Gfp transgenic embryos. Accumulation of hair cells was normal except in tfap2a morphants at 36 hpf (*), which showed a small but significant decrease relative to the control.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dlx3b AB heat shock Prim-5 otic vesicle dorsal region ISH
tfap2am819/m819 standard conditions Prim-5 otic vesicle dorsal region ISH
x24Tg heat shock Prim-5 otic vesicle dorsal region ISH
otx1 AB heat shock Prim-5 otic vesicle ventro-lateral region ISH
tfap2am819/m819 standard conditions Prim-5 otic vesicle ventro-lateral region ISH
x24Tg heat shock Prim-5 otic vesicle ventro-lateral region ISH
pax5 AB heat shock Prim-5 otic vesicle antero-ventral region ISH
AB + MO4-tfap2a standard conditions Prim-5 otic vesicle antero-ventral region ISH
x24Tg heat shock Prim-5 otic vesicle antero-ventral region ISH
pou3f3b AB heat shock Prim-5 otic vesicle postero-medial region ISH
AB + MO4-tfap2a standard conditions Prim-5 otic vesicle postero-medial region ISH
x24Tg heat shock Prim-5 otic vesicle postero-medial region ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO4-tfap2a standard conditions Prim-5 otic vesicle morphology, normal
Prim-15 - Long-pec otic vesicle has normal numbers of parts of type otic vesicle hair cell, normal
Prim-15 - Long-pec otic vesicle hair cell differentiation occurrence, normal
tfap2am819/m819 standard conditions Prim-5 otic vesicle morphology, normal
Prim-15 - Long-pec otic vesicle has normal numbers of parts of type otic vesicle hair cell, normal
Prim-15 - Long-pec otic vesicle hair cell differentiation occurrence, normal
x24Tg heat shock Prim-5 otic vesicle morphology, normal
Prim-15 - Long-pec otic vesicle has normal numbers of parts of type otic vesicle hair cell, normal
Prim-15 - Long-pec otic vesicle hair cell differentiation occurrence, normal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.