ZFIN ID: ZDB-FIG-150504-18
Kantarci et al., 2015 - Tfap2a Promotes Specification and Maturation of Neurons in the Inner Ear through Modulation of Bmp, Fgf and Notch Signaling. PLoS Genetics   11:e1005037 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Term:
Stage Range: Prim-5 to High-pec
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to High-pec

Fig. 2 Tfap2a enhances otic neurogenesis.

(A-L) Cross-sections (medial left, dorsal up) through the otic region just posterior to the utricular macula showing ngn1 expression in +/+ control embryos, hs:tfap2a embryos and tfap2a-/- mutants at the indicated times. Wild-type and hs:tfap2a embryos were heat shocked as indicated in each panel. Overexpression of tfap2a increases the number of neuroblasts in the otic vesicle whereas loss of tfap2a slows down and decreases otic neurogenesis. The outer and inner edges of the otic vesicle are outlined in each image. (M, N) Mean and standard deviation of the total number of ngn1 positive cells in the otic epithelium from 24 to 32 hpf for the genotypes indicated in the color key (counted from serial sections, n = 3–7 ears per time point). Asterisks (*) indicate statistically significant differences between groups indicated by brackets (N) or compared to control embryos (M).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
neurog1 AB heat shock Prim-5 otic vesicle neuroblast (sensu Vertebrata) ISH
Prim-15 otic vesicle neuroblast (sensu Vertebrata) ISH
High-pec Not Detected otic vesicle ISH
tfap2am819/m819 standard conditions Prim-5 otic vesicle neuroblast (sensu Vertebrata) ISH
x24Tg heat shock Prim-5 otic vesicle neuroblast (sensu Vertebrata) ISH
Prim-15 otic vesicle neuroblast (sensu Vertebrata) ISH
High-pec otic vesicle neuroblast (sensu Vertebrata) ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB heat shock Prim-5 otic vesicle has normal numbers of parts of type otic vesicle neuroblast (sensu Vertebrata), normal
Prim-5 otic vesicle neuroblast fate specification occurrence, normal
Prim-15 otic vesicle has normal numbers of parts of type otic vesicle neuroblast (sensu Vertebrata), normal
Prim-15 otic vesicle neuroblast fate specification occurrence, normal
High-pec otic vesicle has normal numbers of parts of type otic vesicle neuroblast (sensu Vertebrata), normal
High-pec otic vesicle neuroblast fate specification occurrence, normal
AB + MO4-tfap2a standard conditions Prim-5 otic vesicle has fewer parts of type otic vesicle neuroblast (sensu Vertebrata), abnormal
Prim-5 otic vesicle neuroblast fate specification decreased occurrence, abnormal
tfap2am819/m819 standard conditions Prim-5 otic vesicle has fewer parts of type otic vesicle neuroblast (sensu Vertebrata), abnormal
Prim-5 otic vesicle neuroblast fate specification decreased occurrence, abnormal
x24Tg heat shock Prim-5 otic vesicle has extra parts of type otic vesicle neuroblast (sensu Vertebrata), abnormal
Prim-5 otic vesicle neuroblast fate specification increased occurrence, abnormal
Prim-15 otic vesicle has extra parts of type otic vesicle neuroblast (sensu Vertebrata), abnormal
Prim-15 otic vesicle neuroblast fate specification increased occurrence, abnormal
High-pec otic vesicle has extra parts of type otic vesicle neuroblast (sensu Vertebrata), abnormal
High-pec otic vesicle neuroblast fate specification increased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.