ZFIN ID: ZDB-FIG-150504-20
Kantarci et al., 2015 - Tfap2a Promotes Specification and Maturation of Neurons in the Inner Ear through Modulation of Bmp, Fgf and Notch Signaling. PLoS Genetics   11:e1005037 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Condition:
Anatomical Term:
Stage: Prim-25
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage Range: Prim-15 to Long-pec

Fig. 4 Tfap2a regulates maturation of SAG neurons.

(A-L) Images of anti-Isl1 antibody staining in control embryos (A-D), tfap2a-/- mutants (E-H) and hs:tfap2a embryos (I-L) at 37 hpf. Control and hs:tfap2a embryos were heat shocked at 24 hpf. Whole-mount specimens (A, E, I) show dorsolateral (anterior to the left) and indicate planes of cross-section in (B-D), (F-H) and (J-L). Cross-sections are oriented with dorsal up and medial to the left. The otic vesicle is outlined in each image. White arrows indicate the SAG population to help distinguish it from other Isl1+ populations present in some sections. (M) Total number of Isl1+ neurons in hs:tfap2a embryos at 30 hpf following heat shock at the indicated times (n = 10–15 each). (N-P) Cross-sections of a hs:tfap2a specimen at 37 hpf following heat shock at 29 hpf. Planes of section are similar to those shown in (I). (Q-S) Cross-sections of a hs:tfap2a embryo stained for TUNEL positive SAG neurons at 42 hpf. (T) Mean and standard deviation of the total number of Isl1+ SAG neurons at the indicated times in +/+ embryos, hs:tfap2a embryos, tfap2a-/- mutants and tfap2a morphants (n = 7–34 embryos per time point). (U) Mean and standard deviation of the total number of TUNEL positive SAG neurons at the indicated times in control embryos and hs:tfap2a embryos (counted from serial sections, n = 3–6 ears per time point). Asterisks (*) indicate statistically significant differences compared to control embryos.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab2-isl IHC AB heat shock Prim-25 statoacoustic (VIII) ganglion neuron
IHC tfap2am819/m819 standard conditions Prim-25 statoacoustic (VIII) ganglion neuron
IHC x24Tg heat shock Prim-25 statoacoustic (VIII) ganglion neuron
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO4-tfap2a standard conditions Prim-25 otic vesicle neurogenesis decreased occurrence, abnormal
Prim-25 statoacoustic (VIII) ganglion has fewer parts of type statoacoustic (VIII) ganglion neuron, abnormal
Prim-25 statoacoustic (VIII) ganglion neuron differentiation decreased rate, abnormal
Long-pec otic vesicle neurogenesis decreased occurrence, abnormal
Long-pec statoacoustic (VIII) ganglion has fewer parts of type statoacoustic (VIII) ganglion neuron, abnormal
Long-pec statoacoustic (VIII) ganglion neuron differentiation decreased rate, abnormal
tfap2am819/m819 standard conditions Prim-25 otic vesicle neurogenesis decreased occurrence, abnormal
Prim-25 statoacoustic (VIII) ganglion has fewer parts of type statoacoustic (VIII) ganglion neuron, abnormal
Prim-25 statoacoustic (VIII) ganglion neuron differentiation decreased rate, abnormal
Long-pec otic vesicle neurogenesis decreased occurrence, abnormal
Long-pec statoacoustic (VIII) ganglion has fewer parts of type statoacoustic (VIII) ganglion neuron, abnormal
Long-pec statoacoustic (VIII) ganglion neuron differentiation decreased rate, abnormal
x24Tg heat shock Prim-15 statoacoustic (VIII) ganglion has extra parts of type statoacoustic (VIII) ganglion neuron, abnormal
Prim-15 statoacoustic (VIII) ganglion apoptotic process increased occurrence, abnormal
Prim-15 statoacoustic (VIII) ganglion neuron apoptotic, abnormal
Prim-25 statoacoustic (VIII) ganglion has extra parts of type statoacoustic (VIII) ganglion neuron, abnormal
Prim-25 statoacoustic (VIII) ganglion apoptotic process increased occurrence, abnormal
Prim-25 statoacoustic (VIII) ganglion neuron apoptotic, abnormal
Long-pec statoacoustic (VIII) ganglion has fewer parts of type statoacoustic (VIII) ganglion neuron, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.