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ZFIN ID: ZDB-FIG-090113-99
Chakraborty et al., 2009 - Loss of ribosomal protein L11 affects zebrafish embryonic development through a p53-dependent apoptotic response. PLoS One   4(1):e4152 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Fig. 4 L11 deficiency results in increased cellular apoptosis mediated by p53.

(A) TUNEL staining at 24 hpf. The morphants show massive apoptosis (blue dots indicate the TUNEL-positive cells) concentrated primarily in the head region compared to a few scattered positive cells in control and wild-type embryos. (B) Histogram showing the number of apoptotic cells in the head region. Values represent the mean of total number of apoptotic cells counted in the head region of three embryos each for the morphant, control, and wild-type embryos. (C-E) Semi-quantitative RT-PCR of p53, p53-target genes, Bcl-2 family genes, atm, gadd45a, myca, and nras transcript levels relative to actin in the morphant, control, and wild-type embryos at 24 hpf. (F) In situ analysis of p53 and mdm2 at 24 hpf. The morphants show increased expression of p53, exclusively in the head region. mdm2 expression, although more ubiquitous, is also increased in the morphants, particularly in the head region. All images are in lateral views with anterior to the top in A and left in F. Scale bars: A, 500 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
atm WT + MO1-rpl11 standard conditions Prim-5 whole organism RTPCR
baxa WT + MO1-rpl11 standard conditions Prim-5 whole organism RTPCR
bbc3 WT + MO1-rpl11 standard conditions Prim-5 whole organism RTPCR
bcl2l1 WT + MO1-rpl11 standard conditions Prim-5 whole organism RTPCR
bik WT + MO1-rpl11 standard conditions Prim-5 whole organism RTPCR
cdkn1a WT + MO1-rpl11 standard conditions Prim-5 whole organism RTPCR
gadd45ab WT standard conditions Prim-5 whole organism RTPCR
mcl1a WT + MO1-rpl11 standard conditions Prim-5 whole organism RTPCR
mdm2 WT standard conditions Prim-5 whole organism ISH
WT + MO1-rpl11 standard conditions Prim-5 head ISH
Prim-5 whole organism RTPCR
myca WT + MO1-rpl11 standard conditions Prim-5 whole organism RTPCR
nras WT + MO1-rpl11 standard conditions Prim-5 whole organism RTPCR
pmaip1 WT + MO1-rpl11 standard conditions Prim-5 whole organism RTPCR
tp53 WT standard conditions Prim-5 whole organism ISH
WT + MO1-rpl11 standard conditions Prim-5 head ISH
Prim-5 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-rpl11 standard conditions Prim-5 apoptotic process increased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One