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ZFIN ID: ZDB-FIG-090113-102
Chakraborty et al., 2009 - Loss of ribosomal protein L11 affects zebrafish embryonic development through a p53-dependent apoptotic response. PLoS One   4(1):e4152 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage Range: Sphere to Long-pec

Fig. S2 Time-course expression analysis of p53 and its target genes. Semi-quantitative RT-PCR of p53, p53 target genes (p21, mdm2) and apoptotic markers (bax and puma) transcript levels relative to actin in the morphants and wild-type embryos at 4, 8, 12, and 48 hpf.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
baxa WT + MO1-rpl11 standard conditions Sphere whole organism RTPCR
75%-epiboly whole organism RTPCR
5-9 somites whole organism RTPCR
Long-pec whole organism RTPCR
bbc3 WT + MO1-rpl11 standard conditions Sphere whole organism RTPCR
75%-epiboly whole organism RTPCR
5-9 somites whole organism RTPCR
Long-pec whole organism RTPCR
cdkn1a WT + MO1-rpl11 standard conditions Sphere whole organism RTPCR
75%-epiboly whole organism RTPCR
5-9 somites whole organism RTPCR
Long-pec whole organism RTPCR
mdm2 WT + MO1-rpl11 standard conditions Sphere whole organism RTPCR
75%-epiboly whole organism RTPCR
5-9 somites whole organism RTPCR
Long-pec whole organism RTPCR
tp53 WT + MO1-rpl11 standard conditions Sphere whole organism RTPCR
75%-epiboly whole organism RTPCR
5-9 somites whole organism RTPCR
Long-pec whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One