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ZFIN ID: ZDB-FIG-090113-98
Chakraborty et al., 2009 - Loss of ribosomal protein L11 affects zebrafish embryonic development through a p53-dependent apoptotic response. PLoS One   4(1):e4152 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-5

Fig. 3 The L11-deficient embryos exhibit reduced abundance of neuronal progenitor cells and aberrant expression of transcription factors.

(A) Expression of otx2 at 24 hpf. The morphants show a reduction in total abundance of otx2, but its pattern of expression is same as that of wild-type embryos. The inset shows the dorsal view of the midbrain region. (B) krox20 expression at the hindbrain in 24 hpf embryos. The morphants show a proper hindbrain patterning compared to the wild-type embryos. (C) Expression of shh in morphants and wild-type embryos at 24 hpf. The morphants show a marginal reduction in the forebrain midbrain boundary (fmb, inset in C), but floor plate (fp) expression is unaffected. (D) Expression of pax6a in morphants and wild-type embryos at 24 hpf. The morphants show a reduction in total abundance of pax6a, but its pattern of expression is same as that of wild-type embryos (E) The morphants show an increased level of pax2 expression compared to wild-type embryos. The midbrain hindbrain boundary (mhb) expression of pax2 is short and wide in the morphants. pax2 expression at the optic stalk and the otic capsules is also increased in the morphants. All images are in lateral views with anterior to the left. Scale bar: 250 μm. hb, hindbrain; dd; dorsal diencephalon; t, telencephalon; r3, rhombomere 3; r5, rhombomere 5; fmb, forebrain-midbrain boundary; os, optic stalk; mhb, midbrain-hindbrain boundary; oc, otic capsule; sn, spinal chord neurons.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
egr2b WT + MO1-rpl11 standard conditions Prim-5 rhombomere 3 ISH
Prim-5 rhombomere 5 ISH
otx2b WT + MO1-rpl11 standard conditions Prim-5 midbrain ISH
pax2a WT + MO1-rpl11 standard conditions Prim-5 midbrain hindbrain boundary ISH
Prim-5 optic stalk ISH
Prim-5 otic vesicle ISH
pax6a WT + MO1-rpl11 standard conditions Prim-5 diencephalon ISH
Prim-5 hindbrain ISH
Prim-5 telencephalon ISH
shha WT + MO1-rpl11 standard conditions Prim-5 floor plate ISH
Prim-5 forebrain midbrain boundary ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One