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ZFIN ID: ZDB-FIG-090113-96
Chakraborty et al., 2009 - Loss of ribosomal protein L11 affects zebrafish embryonic development through a p53-dependent apoptotic response. PLoS One   4(1):e4152 Full text @ PLoS One
Knockdown Reagent:
Anatomical Term:
Stage: Prim-5
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to Day 4

Fig. 1 L11 deficiency causes morphological defects in zebrafish.

(A) Lateral views of morphants (rpl11 MO, upper panel), controls (control MO, middle panel), and wild-type embryos (WT, lower panel) at 24 hpf. The morphants are smaller in size to the control embryos and the yolk sac extension is incomplete (black solid line). The right hand panel shows a close-up of the head region. The morphants display deformities in various parts of the brain, such as the midbrain-hindbrain boundary (filled black triangle), hindbrain (asterisk), and otolith (arrow). (B) Morphological observation of morphants and wild-type embryos at 4 dpf. The morphants have a smaller head and eyes, severe edema of the heart (arrow, upper panel), and incomplete yolk consumption with no gut (white solid line, upper panel) compared to wild-type embryos. (C) Western blot analysis of L11 in the morphants and control embryos with fibrillarin as a loading control. All images are anterior to the left. Scale bars: A, 500 μm (left panel), 100 μm (right panel); B, 500 μm. e, eye; g, gut.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
rpl11 WT control Prim-5 whole organism WB
WT + MO1-rpl11 standard conditions Prim-5 whole organism WB
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-rpl11 standard conditions Prim-5 extension decreased size, abnormal
Prim-5 eye pigmentation delayed, abnormal
Prim-5 forebrain increased size, abnormal
Prim-5 head opaque, abnormal
Prim-5 hindbrain malformed, abnormal
Prim-5 inner ear morphology, abnormal
Prim-5 midbrain-hindbrain boundary structural organization disrupted, abnormal
Prim-5 otolith deformed, abnormal
Prim-5 whole organism decreased length, abnormal
Day 4 eye decreased size, abnormal
Day 4 gut aplastic, abnormal
Day 4 head decreased size, abnormal
Day 4 pericardium edematous, abnormal
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One