ZFIN ID: ZDB-FIG-170210-7
Wang et al., 2016 - The somite-secreted factor Maeg promotes zebrafish embryonic angiogenesis. Oncotarget   7(47):77749-77763 Full text @ Oncotarget
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-15
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage: Prim-15

Fig. 7

The phenotype of maeg and itgb1 loss-of-function involves Notch signaling. A. Whole mount in situ hybridization analysis of zebrafish embryos using antisense notch1a, notch1b, notch2, notch3, jag1a, jag1b, and jag2b probes. 30 hpf, lateral view. Blue arrowheads indicate the notochord position. Red arrowheads indicate the trunk vessel position. B. Confocal images of ISVs in control embryos, maeg morphants, maeg morphants treated with DAPT, and maeg morphants coinjected with dll4 MO using Tg(kdrl:EGFP) transgenic embryos. The red and white dash lines indicate the position of dorsal roof and horizontal myoseptum respectively. C. The statistics of ISV length in 30 hpf control embryos, maeg morphants, maeg morphants treated with DAPT, and maeg morphants coinjected with dll4 MO. One-Way ANOVA; ***,P<0.001. D. Confocal imaging analysis of endothelial numbers of ISVs in 30 hpf control embryos, maeg morphants, maeg morphants treated with DAPT, and maeg morphants coinjected with dll4 MO using Tg(fli1a:EGFP) transgenic embryos. Nuclei of ISVs are numbered. E. Quantification of ECs nuclei number in ISV. Measurements were made from three adjacent ISVs (over yolk) per embryo from 3 independent experiments. One-Way ANOVA; ***,P<0.001. F. Confocal images of ISVs in control embryos, itgb1a morphants, and itgb1a morphants coinjected with dll4 MO using Tg(kdrl:ras-mCherry::fli1a:nEGFP) transgenic line at 30 hpf. G. Quantification of ECs nuclei number in ISV. Measurements were made from three adjacent ISVs (over yolk) per embryo from 3 independent experiments. One-Way ANOVA; ***,P<0.001. H. The statistics of ISV length. One-Way ANOVA; ***,P<0.001.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP s843Tg standard conditions Prim-15 intersegmental vessel IFL
Prim-15 trunk vasculature IFL
s843Tg + MO1-egfl6 standard conditions Prim-15 intersegmental vessel IFL
Prim-15 trunk vasculature IFL
s843Tg + MO1-egfl6 chemical treatment by environment: DAPT Prim-15 intersegmental vessel IFL
Prim-15 trunk vasculature IFL
s843Tg + MO1-egfl6 + MO3-dll4 standard conditions Prim-15 intersegmental vessel IFL
Prim-15 trunk vasculature IFL
s916Tg; y7Tg standard conditions Prim-15 endothelial cell nucleus IFL
Prim-15 intersegmental vessel endothelial cell IFL
s916Tg; y7Tg + MO1-itgb1a standard conditions Prim-15 endothelial cell nucleus IFL
Prim-15 intersegmental vessel endothelial cell IFL
s916Tg; y7Tg + MO1-itgb1a + MO3-dll4 standard conditions Prim-15 endothelial cell nucleus IFL
Prim-15 intersegmental vessel endothelial cell IFL
y7Tg standard conditions Prim-15 endothelial cell nucleus IFL
Prim-15 intersegmental vessel endothelial cell IFL
y7Tg + MO1-egfl6 standard conditions Prim-15 endothelial cell nucleus IFL
Prim-15 intersegmental vessel endothelial cell IFL
y7Tg + MO1-egfl6 chemical treatment by environment: DAPT Prim-15 endothelial cell nucleus IFL
Prim-15 intersegmental vessel endothelial cell IFL
y7Tg + MO1-egfl6 + MO3-dll4 standard conditions Prim-15 endothelial cell nucleus IFL
Prim-15 intersegmental vessel endothelial cell IFL
jag1a AB standard conditions Prim-15 trunk ISH
egfl6zf774/zf774(AB) standard conditions Prim-15 trunk ISH
jag1b AB standard conditions Prim-15 trunk ISH
egfl6zf774/zf774(AB) standard conditions Prim-15 trunk ISH
jag2b AB standard conditions Prim-15 trunk ISH
egfl6zf774/zf774(AB) standard conditions Prim-15 trunk ISH
mCherry s916Tg; y7Tg standard conditions Prim-15 intersegmental vessel IFL
Prim-15 trunk vasculature IFL
s916Tg; y7Tg + MO1-itgb1a standard conditions Prim-15 intersegmental vessel IFL
Prim-15 trunk vasculature IFL
s916Tg; y7Tg + MO1-itgb1a + MO3-dll4 standard conditions Prim-15 intersegmental vessel IFL
Prim-15 trunk vasculature IFL
notch1a AB standard conditions Prim-15 trunk ISH
egfl6zf774/zf774(AB) standard conditions Prim-15 trunk ISH
notch1b AB standard conditions Prim-15 trunk ISH
egfl6zf774/zf774(AB) standard conditions Prim-15 trunk ISH
notch2 AB standard conditions Prim-15 trunk ISH
egfl6zf774/zf774(AB) standard conditions Prim-15 trunk ISH
notch3 AB standard conditions Prim-15 trunk ISH
egfl6zf774/zf774(AB) standard conditions Prim-15 trunk ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
egfl6zf774/zf774(AB) standard conditions Prim-15 trunk notch1a expression increased amount, abnormal
egfl6zf774/zf774(AB) standard conditions Prim-15 trunk notch1b expression increased amount, abnormal
s843Tg + MO1-egfl6 standard conditions Prim-15 intersegmental vessel decreased length, abnormal
s843Tg + MO1-egfl6 chemical treatment by environment: DAPT Prim-15 intersegmental vessel decreased length, ameliorated
s843Tg + MO1-egfl6 + MO3-dll4 standard conditions Prim-15 intersegmental vessel decreased length, ameliorated
s916Tg; y7Tg + MO1-itgb1a standard conditions Prim-15 intersegmental vessel decreased length, abnormal
Prim-15 intersegmental vessel endothelial cell decreased amount, abnormal
s916Tg; y7Tg + MO1-itgb1a + MO3-dll4 standard conditions Prim-15 intersegmental vessel decreased length, ameliorated
Prim-15 intersegmental vessel endothelial cell amount, ameliorated
y7Tg + MO1-egfl6 standard conditions Prim-15 intersegmental vessel endothelial cell decreased amount, abnormal
y7Tg + MO1-egfl6 chemical treatment by environment: DAPT Prim-15 intersegmental vessel endothelial cell amount, ameliorated
y7Tg + MO1-egfl6 + MO3-dll4 standard conditions Prim-15 intersegmental vessel endothelial cell amount, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal Oncotarget for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Oncotarget