ZFIN ID: ZDB-FIG-170210-6
Wang et al., 2016 - The somite-secreted factor Maeg promotes zebrafish embryonic angiogenesis. Oncotarget   7(47):77749-77763 Full text @ Oncotarget
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Prim-15
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to Prim-15

Fig. 6

Maeg regulates angiogenesis dependent on RGD domain. A. The diagram of Maeg protein with RGD domain and Maeg protein with RGD domain mutated to RGE domain (MaegΔRGD). B. Confocal images of ISVs in control embryos, maeg morphants, maeg morphants treated with DAPT, and maeg morphants coinjected with dll4 MO using Tg(kdrl:EGFP) transgenic embryos. C. Percentage of embryos with ISV defect in each group. χ2 test; ***, P<0.001. D, E. Phosphorylation levels of ERK and Akt determined by Western blot analysis in maeg loss- and gain-of-function embryos. F. Confocal images of ISVs in control embryos, maeg mutants, itgb1a morphants, and itgb1a morphants coinjected with maeg mRNA using Tg(kdrl:EGFP) transgenic embryos. G. Percentage of embryos with ISV defect in each group.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP egfl6zf774/zf774 ; s843Tg standard conditions Prim-15 trunk vasculature IFL
s843Tg standard conditions Prim-15 trunk vasculature IFL
s843Tg + MO1-itgb1a standard conditions Prim-15 trunk vasculature IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab11-mapk WB egfl6zf774/zf774(AB) standard conditions Prim-5 - Prim-15 whole organism
Ab20-mapk WB egfl6zf774/zf774(AB) standard conditions Prim-5 - Prim-15 whole organism
Phenotype Details
Fish Conditions Stage Phenotype
egfl6zf774/zf774(AB) standard conditions Prim-5 - Prim-15 whole organism ab11-mapk labeling decreased amount, abnormal
egfl6zf774/zf774(AB) standard conditions Prim-5 - Prim-15 whole organism ab20-mapk labeling decreased amount, abnormal
egfl6zf774/zf774 ; s843Tg standard conditions Prim-15 dorsal longitudinal anastomotic vessel blood vessel morphogenesis decreased occurrence, abnormal
Prim-15 intersegmental vessel decreased length, abnormal
Prim-15 sprouting angiogenesis decreased occurrence, abnormal
s843Tg + MO1-itgb1a standard conditions Prim-15 dorsal longitudinal anastomotic vessel blood vessel morphogenesis decreased occurrence, abnormal
Prim-15 intersegmental vessel decreased length, abnormal
Prim-15 sprouting angiogenesis decreased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Oncotarget for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Oncotarget