ZFIN ID: ZDB-FIG-150511-13 |
Genes: | |
---|---|
Fish: | |
Anatomical Terms: | |
Stage: | Day 6 |
Fig. 6 High-fat feed increases expression of apolipoproteins in larval gut. (A) Representative images of mRNA expression as observed by ISH of the zebrafish apoA-I, apoB, apoE and apoA-IV genes in 6-dpf nacre-/- larvae. Larvae have either never eaten exogenous food (Unfed), or have been fed a high-fat, 5% chicken egg yolk meal for 4 hours (Fed). ISH was performed in triplicate with n≥5 larvae; no staining was observed in sense probed (supplementary material Figs S1-S4). (B) A high-fat meal increases zebrafish apoA-IVa, apoA-IVb.2, and apoA-IVb.3 mRNA expression in the gut (intestine, liver, pancreas). Real-time PCR quantification of apolipoprotein transcriptional response in the 6-dpf zebrafish gut to 1, 2, 3 or 4 hours of a 10% chicken egg yolk feed (n=3; 10 pooled larval guts per experiment). Groups with the same letter are not significantly different (one-way ANOVA, P<0.05, n=3). |
Gene | Antibody | Fish | Conditions | Stage | Qualifier | Anatomy | Assay |
---|---|---|---|---|---|---|---|
apoa1a | mitfaw2/w2 | control | Day 6 | intestine | ISH | ||
apoa1b | mitfaw2/w2 | control | Day 6 | intestine | ISH | ||
Day 6 | liver | ISH | |||||
apoa4a | mitfaw2/w2 | control | Day 6 | intestine | ISH | ||
apoa4b.1 | mitfaw2/w2 | control | Day 6 | intestine | ISH | ||
Day 6 | liver | ISH | |||||
apoa4b.2 | mitfaw2/w2 | control | Day 6 | intestine | ISH | ||
apoa4b.3 | mitfaw2/w2 | control | Day 6 | intestine | ISH | ||
apoba | mitfaw2/w2 | control | Day 6 | intestine | ISH | ||
Day 6 | liver | ISH | |||||
apobb.1 | mitfaw2/w2 | control | Day 6 | intestine | ISH | ||
Day 6 | liver | ISH | |||||
apobb.2 | mitfaw2/w2 | control | Day 6 | Not Detected | intestine | ISH | |
Day 6 | Not Detected | liver | ISH | ||||
apoea | mitfaw2/w2 | control | Day 6 | intestine | ISH | ||
apoeb | mitfaw2/w2 | control | Day 6 | intestine | ISH |
Acknowledgments: | ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech. |
---|