ZFIN ID: ZDB-FIG-150511-9 |
Genes: | |
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Fish: | |
Anatomical Terms: | |
Stage Range: | 75%-epiboly to Day 6 |
Fig. 2 Developmental mRNA expression patterns of apoA-Ia and apoA-Ib. In situ hybridization of apoA-Ia and apoA-Ib during gastrulation [80-100% epiboly (E)], somitogenesis [15-20 somite (S)], and daily until 6 dpf. Both genes localize to the YSL from somitogenesis through 5 dpf, apoA-Ia localizes to the intestine (I) at 6 dpf, and apoA-Ib localizes strongly to the liver (L) and weakly to the intestine at 6 dpf. All zebrafish are wild type except 6-dpf larvae, which are nacre-/-. Larvae studied at 2-5 dpf were treated with PTU to prevent pigment formation. No signal was observed for either gene at the eight-cell stage or blastulation (supplementary material Fig. S1). Experiments were performed three times for each gene at each stage with ne5 embryos or larvae per probe per experiment. |
Gene | Antibody | Fish | Conditions | Stage | Anatomy | Assay |
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apoa1a | AB | control | 75%-epiboly to Bud | yolk syncytial layer | ISH | |
14-19 somites to 20-25 somites | yolk syncytial layer | ISH | ||||
Prim-5 | yolk syncytial layer | ISH | ||||
Long-pec | yolk syncytial layer | ISH | ||||
Protruding-mouth | yolk syncytial layer | ISH | ||||
Day 4 | yolk syncytial layer | ISH | ||||
Day 5 | yolk syncytial layer | ISH | ||||
mitfaw2/w2 | standard conditions | Day 6 | intestine | ISH | ||
apoa1b | AB | control | 75%-epiboly to Bud | yolk syncytial layer | ISH | |
14-19 somites to 20-25 somites | yolk syncytial layer | ISH | ||||
Prim-5 | yolk syncytial layer | ISH | ||||
Long-pec | yolk syncytial layer | ISH | ||||
Protruding-mouth | yolk syncytial layer | ISH | ||||
Day 4 | yolk syncytial layer | ISH | ||||
Day 5 | yolk syncytial layer | ISH | ||||
mitfaw2/w2 | standard conditions | Day 6 | intestine | ISH | ||
Day 6 | liver | ISH |
Acknowledgments: | ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech. |
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