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ZFIN ID: ZDB-FIG-150511-12
Otis et al., 2015 - Zebrafish as a model for apolipoprotein biology: Comprehensive expression analysis and a role for ApoA-IV in regulating food intake. Disease models & mechanisms   8(3):295-309 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 14-19 somites to Day 6

Fig. 5

The zebrafish apoA-IV genes have unique expression patterns in the YSL, intestine and liver. Developmental mRNA expression dynamics of the zebrafish apoA-IV genes, as measured by ISH, during somitogenesis [15-20 somite (S)] and at 1-6 dpf. All four apoA-IV genes showed earliest mRNA expression at 15-20 S in the YSL; the mRNAs localize to distinct subregions of the YSL until 5 dpf. All four genes are expressed in the intestine (I) at 4-6 dpf, with weak intestinal expression observed for apoA-IVa, and apoA-IVb.3 is expressed in the liver (L) at 4-6 dpf. No expression was observed for the apoA-IV genes at the eight-cell stage, 30% epiboly or 80-100% epiboly (supplementary material Fig. S4). Larvae from 15-20 S to 5 dpf are wild type (2-5 dpf treated with PTU; 6-dpf larvae are nacre-/-. Experiments were performed in triplicate with ne5 embryos or larvae per probe in every experiment.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
apoa4a AB control 14-19 somites to 20-25 somites yolk syncytial layer ISH
Prim-5 yolk syncytial layer ISH
Long-pec yolk syncytial layer ISH
Protruding-mouth yolk syncytial layer ISH
Day 4 intestine ISH
Day 4 yolk syncytial layer ISH
Day 5 intestine ISH
Day 5 yolk syncytial layer ISH
mitfaw2/w2 standard conditions Day 6 intestine ISH
apoa4b.1 AB control 14-19 somites to 20-25 somites yolk syncytial layer ISH
Prim-5 yolk syncytial layer ISH
Long-pec yolk syncytial layer ISH
Protruding-mouth yolk syncytial layer ISH
Day 4 intestine ISH
Day 4 yolk syncytial layer ISH
Day 5 intestine ISH
Day 5 yolk syncytial layer ISH
mitfaw2/w2 standard conditions Day 6 intestine ISH
apoa4b.2 AB control 14-19 somites to 20-25 somites yolk syncytial layer ISH
Prim-5 yolk syncytial layer ISH
Long-pec yolk syncytial layer ISH
Protruding-mouth yolk syncytial layer ISH
Day 4 intestine ISH
Day 4 yolk syncytial layer ISH
Day 5 intestine ISH
Day 5 yolk syncytial layer ISH
mitfaw2/w2 standard conditions Day 6 intestine ISH
apoa4b.3 AB control 14-19 somites to 20-25 somites yolk syncytial layer ISH
Prim-5 yolk syncytial layer ISH
Long-pec yolk syncytial layer ISH
Protruding-mouth yolk syncytial layer ISH
Day 4 intestine ISH
Day 4 liver ISH
Day 4 yolk syncytial layer ISH
Day 5 intestine ISH
Day 5 liver ISH
Day 5 yolk syncytial layer ISH
mitfaw2/w2 standard conditions Day 6 intestine ISH
Day 6 liver ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.