header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-150511-11
Otis et al., 2015 - Zebrafish as a model for apolipoprotein biology: Comprehensive expression analysis and a role for ApoA-IV in regulating food intake. Disease models & mechanisms   8(3):295-309 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 30%-epiboly to Day 6

Fig. 4

apoEa localization to the YSL and intestine contrasts with widespread expression of apoEb. apoEa and apoEb mRNA expression observed by ISH during blastulation [30% epiboly (E)], gastrulation (80% E), somitogenesis [15-20 somite (S)] and 1-6 dpf. apoEa mRNA is expressed in distinct subregions of the YSL at somitogenesis and 1 dpf, and ubiquitously throughout the YSL from 2-5 dpf. At 6 dpf (but not 5 dpf), apoEa is observed in the intestine (I). apoEb is expressed not only throughout the YSL (30% E to 5 dpf), but also in the tail bud (15-20 S and 1 dpf), the apical ectodermal ridge (AER) (1-6 dpf), the head (H) (1-6 dpf), mouth and nose orifices (O) (2-6 dpf), the find buds (FB) (2-6 dpf), pharynx (P) (2-6 dpf), gill arches (GA) (3-6 dpf), periderm (4-6 dpf), retina (R) (4-6 dpf), intestine (6 dpf) and swim bladder (6 dpf). No expression of apoEa was observed during blastulation or gastrulation, or for either gene at the eight-cell stage (supplementary material Fig. S3). Zebrafish are wild type, except for 6-dpf larvae which are nacre-/-; 1-6 dpf larvae were treated with hydrogen peroxide to remove pigmentation. ISH was performed in triplicate with ne5 embryos or larvae per probe per experiment.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
apoea AB control 14-19 somites to 20-25 somites yolk syncytial layer ISH
Prim-5 yolk syncytial layer ISH
Long-pec yolk syncytial layer ISH
Protruding-mouth yolk syncytial layer ISH
Day 4 yolk syncytial layer ISH
Day 5 yolk syncytial layer ISH
mitfaw2/w2 control Day 6 intestine ISH
apoeb AB control 30%-epiboly yolk syncytial layer ISH
75%-epiboly to Bud yolk syncytial layer ISH
14-19 somites to 20-25 somites tail bud ISH
14-19 somites to 20-25 somites yolk syncytial layer ISH
Prim-5 head ISH
Prim-5 tail bud ISH
Prim-5 yolk syncytial layer ISH
Long-pec apical ectodermal ridge ISH
Long-pec fin bud ISH
Long-pec head ISH
Long-pec mouth ISH
Long-pec peripheral olfactory organ ISH
Long-pec pharynx ISH
Long-pec yolk syncytial layer ISH
Protruding-mouth apical ectodermal ridge ISH
Protruding-mouth fin bud ISH
Protruding-mouth head ISH
Protruding-mouth mouth ISH
Protruding-mouth peripheral olfactory organ ISH
Protruding-mouth pharyngeal arch ISH
Protruding-mouth pharynx ISH
Protruding-mouth yolk syncytial layer ISH
Day 4 apical ectodermal ridge ISH
Day 4 epidermal superficial stratum ISH
Day 4 fin bud ISH
Day 4 head ISH
Day 4 mouth ISH
Day 4 peripheral olfactory organ ISH
Day 4 pharyngeal arch ISH
Day 4 pharynx ISH
Day 4 retina ISH
Day 4 yolk syncytial layer ISH
Day 5 apical ectodermal ridge ISH
Day 5 epidermal superficial stratum ISH
Day 5 fin bud ISH
Day 5 head ISH
Day 5 mouth ISH
Day 5 peripheral olfactory organ ISH
Day 5 pharyngeal arch ISH
Day 5 pharynx ISH
Day 5 retina ISH
Day 5 yolk syncytial layer ISH
mitfaw2/w2 control Day 6 apical ectodermal ridge ISH
Day 6 epidermal superficial stratum ISH
Day 6 fin bud ISH
Day 6 head ISH
Day 6 intestine ISH
Day 6 mouth ISH
Day 6 peripheral olfactory organ ISH
Day 6 pharyngeal arch ISH
Day 6 pharynx ISH
Day 6 retina ISH
Day 6 swim bladder ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.