ZFIN ID: ZDB-FIG-130930-1
Fodor et al., 2013 - Full transcriptome analysis of early dorsoventral patterning in zebrafish. PLoS One   8(7):e70053 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: 30%-epiboly

Fig. 2

Expression of pan-mesodermal candidate genes.

The majority of the identified genes show early dorsal expression in wild type embryos and lack thereof in ich controls. This is followed by a second wave of circumferential, mesodermal expression, which could be detected in both genetic backgrounds. An interesting exception is flh, which shows a somewhat counterintuitive expression dynamics: a very strong circumferential expression (complemented by strong dorsal signal in wild type embryos) can be detected as early as late sphere stage (Ei, Eii), but this expression becomes significantly weaker by 30% epiboly, except in the dorsal side of wild type embryos (Eiii, Eiv). Sphere stage embryos are shown from a lateral view, whereas ~30% epiboly stage embryos are presented from an animal view (dorsal to the right in both cases).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
arl4ab EKW control 30%-epiboly margin ISH
Sphere whole organism dorsal region ISH
ctnnb2p1/p1 standard conditions 30%-epiboly margin ISH
Sphere Not Detected whole organism dorsal region ISH
dkk1b EKW control 30%-epiboly margin ISH
Sphere whole organism dorsal region ISH
ctnnb2p1/p1 standard conditions 30%-epiboly margin ISH
Sphere Not Detected whole organism dorsal region ISH
fgf8a EKW control 30%-epiboly margin ISH
Sphere whole organism dorsal region ISH
ctnnb2p1/p1 standard conditions 30%-epiboly margin ISH
Sphere Not Detected whole organism dorsal region ISH
fgf17 EKW control 30%-epiboly margin ISH
Sphere whole organism dorsal region ISH
ctnnb2p1/p1 standard conditions 30%-epiboly margin ISH
Sphere Not Detected whole organism dorsal region ISH
foxa3 EKW control 30%-epiboly margin ISH
Sphere whole organism dorsal region ISH
ctnnb2p1/p1 standard conditions 30%-epiboly margin ISH
Sphere Not Detected whole organism dorsal region ISH
foxd5 EKW control 30%-epiboly margin ISH
Sphere whole organism dorsal region ISH
ctnnb2p1/p1 standard conditions 30%-epiboly margin ISH
Sphere Not Detected whole organism dorsal region ISH
hnf1ba EKW control 30%-epiboly margin ISH
Sphere whole organism dorsal region ISH
ctnnb2p1/p1 standard conditions 30%-epiboly margin ISH
Sphere Not Detected whole organism dorsal region ISH
ism1 EKW control 30%-epiboly margin ISH
Sphere whole organism dorsal region ISH
ctnnb2p1/p1 standard conditions 30%-epiboly margin ISH
Sphere Not Detected whole organism dorsal region ISH
lft1 EKW control 30%-epiboly margin ISH
Sphere whole organism dorsal region ISH
ctnnb2p1/p1 standard conditions 30%-epiboly margin ISH
Sphere Not Detected whole organism dorsal region ISH
mixl1 EKW control 30%-epiboly margin ISH
Sphere whole organism dorsal region ISH
ctnnb2p1/p1 standard conditions 30%-epiboly margin ISH
Sphere Not Detected whole organism dorsal region ISH
mst1 EKW control 30%-epiboly margin ISH
Sphere whole organism dorsal region ISH
ctnnb2p1/p1 standard conditions 30%-epiboly margin ISH
Sphere Not Detected whole organism dorsal region ISH
noto EKW control 30%-epiboly margin ISH
Sphere whole organism dorsal region ISH
ctnnb2p1/p1 standard conditions 30%-epiboly margin ISH
Sphere whole organism dorsal region ISH
notum1a EKW control 30%-epiboly margin ISH
Sphere whole organism dorsal region ISH
ctnnb2p1/p1 standard conditions 30%-epiboly margin ISH
Sphere Not Detected whole organism dorsal region ISH
osr1 EKW control 30%-epiboly margin ISH
Sphere whole organism dorsal region ISH
ctnnb2p1/p1 standard conditions 30%-epiboly margin ISH
Sphere Not Detected whole organism dorsal region ISH
ppp1r3ca EKW control 30%-epiboly margin ISH
Sphere whole organism dorsal region ISH
ctnnb2p1/p1 standard conditions 30%-epiboly margin ISH
Sphere whole organism dorsal region ISH
rnd1b EKW control 30%-epiboly margin ISH
Sphere whole organism dorsal region ISH
ctnnb2p1/p1 standard conditions 30%-epiboly margin ISH
Sphere whole organism dorsal region ISH
sp5l EKW control 30%-epiboly margin ISH
Sphere whole organism dorsal region ISH
ctnnb2p1/p1 standard conditions 30%-epiboly margin ISH
Sphere Not Detected whole organism dorsal region ISH
spry2 EKW control 30%-epiboly margin ISH
Sphere whole organism dorsal region ISH
ctnnb2p1/p1 standard conditions 30%-epiboly margin ISH
Sphere Not Detected whole organism dorsal region ISH
spry4 EKW control 30%-epiboly margin ISH
Sphere whole organism dorsal region ISH
ctnnb2p1/p1 standard conditions 30%-epiboly margin ISH
Sphere Not Detected whole organism dorsal region ISH
tbxta EKW control 30%-epiboly margin ISH
Sphere whole organism dorsal region ISH
ctnnb2p1/p1 standard conditions 30%-epiboly margin ISH
Sphere Not Detected whole organism dorsal region ISH
zic2a EKW control 30%-epiboly margin ISH
Sphere whole organism dorsal region ISH
ctnnb2p1/p1 standard conditions 30%-epiboly margin ISH
Sphere Not Detected whole organism dorsal region ISH
zic3 EKW control 30%-epiboly margin ISH
Sphere whole organism dorsal region ISH
ctnnb2p1/p1 standard conditions 30%-epiboly margin ISH
Sphere Not Detected whole organism dorsal region ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One