ZFIN ID: ZDB-FIG-090331-10
Nelson et al., 2009 - Retinal homeobox 1 is required for retinal neurogenesis and photoreceptor differentiation in embryonic zebrafish. Developmental Biology   328(1):24-39 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-5 to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 4 Expression of ath5 and pax6 but not rx2 is disrupted in rx1-depleted embryos. (A–C) Embryos fixed at 25 hpf and processed for in situ hybridization with an ath5 probe show strong retinal labeling in control embryos (A), no labeling in rx1-depleted embryos, and a normal ath5 expression pattern in rx2-depleted embryos. (D–F) Embryos fixed at 34 hpf and processed for in situ hybridization with a pax6 probe, showing labeling in the retina, lens and forebrain of control embryos (D), labeling in lens and forebrain only in rx1-depleted embryos (E), and a normal pattern of expression of pax6 in rx2-depleted embryos (F). (G–H) Sections obtained from embryos fixed at 53 hpf following injection with control-MOs (G) or rx1-depleting MOs (H) and hybridized with a probe for pax6, showing pax6 expression in the retinas of the morphants, albeit in a pattern reflecting a delay in neurogenesis. (I–L) Embryos processed at 34 hpf as whole mounts (I, J) or at 72 hpf as cryosections (K, L), for in situ hybridization with an rx2 probe, following injection with control-MOs (G) or rx1-depleting MOs, showing that rx2 is not regulated by rx1. le = lens; gcl = ganglion cell layer; inl = inner nuclear layer; onl = outer nuclear layer; cm = ciliary margin; scale bar in A and G = 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
atoh7 WT control Prim-5 optic cup ISH
WT + MO2-rx1 + MO3-rx1 standard conditions Prim-5 Not Detected optic cup ISH
WT + MO2-rx2 + MO3-rx2 standard conditions Prim-5 optic cup ISH
pax6a WT control Prim-15 forebrain ISH
Prim-15 lens ISH
Prim-15 retina ISH
Long-pec retina ISH
WT + MO2-rx1 + MO3-rx1 standard conditions Prim-15 forebrain ISH
Prim-15 lens ISH
Long-pec retina ISH
WT + MO2-rx2 + MO3-rx2 standard conditions Prim-15 forebrain ISH
Prim-15 lens ISH
Prim-15 retina ISH
rx2 WT control Prim-15 retina ISH
Protruding-mouth ciliary marginal zone ISH
Protruding-mouth retinal inner nuclear layer ISH
Protruding-mouth retinal outer nuclear layer ISH
WT + MO2-rx1 + MO3-rx1 standard conditions Prim-15 retina ISH
Protruding-mouth retina ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-rx1 + MO3-rx1 standard conditions Long-pec neural retina development delayed, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 328(1), Nelson, S.M., Park, L., and Stenkamp, D.L., Retinal homeobox 1 is required for retinal neurogenesis and photoreceptor differentiation in embryonic zebrafish, 24-39, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.