ZFIN ID: ZDB-FIG-090331-15
Nelson et al., 2009 - Retinal homeobox 1 is required for retinal neurogenesis and photoreceptor differentiation in embryonic zebrafish. Developmental Biology   328(1):24-39 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Protruding-mouth

Fig. 9 Temporally-selective rx2 depletion results in a selective reduction in the expression of rod and red cone opsins and rod and cone transducins. Embryos were injected with the 5-mispair control MO (A, C, E, G, I, K) or an rx2-targeting MO cocktail (B, D, F, H, J, L) and were processed as whole mounts for in situ hybridization with cRNA probes corresponding to red cone opsin (A, B), rod opsin (C, D), blue cone opsin (E, F), rod transducin alpha subunit (gnat1; G, H), UV cone opsin (I, J), or cone transducin alpha subunit (gnat2; K, L). Scale bar = 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gnat1 WT control Protruding-mouth retinal photoreceptor layer ISH
WT + MO2-rx2 + MO3-rx2 standard conditions Protruding-mouth retinal photoreceptor layer ISH
gnat2 WT control Protruding-mouth retinal photoreceptor layer ISH
WT + MO2-rx2 + MO3-rx2 standard conditions Protruding-mouth retinal photoreceptor layer ISH
opn1lw1 WT control Protruding-mouth retinal photoreceptor layer ISH
WT + MO2-rx2 + MO3-rx2 standard conditions Protruding-mouth retinal photoreceptor layer ISH
opn1sw1 WT control Protruding-mouth retinal photoreceptor layer ISH
WT + MO2-rx2 + MO3-rx2 standard conditions Protruding-mouth retinal photoreceptor layer ISH
opn1sw2 WT control Protruding-mouth retinal photoreceptor layer ISH
WT + MO2-rx2 + MO3-rx2 standard conditions Protruding-mouth retinal photoreceptor layer ISH
rho WT control Protruding-mouth retinal photoreceptor layer ISH
WT + MO2-rx2 + MO3-rx2 standard conditions Protruding-mouth retinal photoreceptor layer ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 328(1), Nelson, S.M., Park, L., and Stenkamp, D.L., Retinal homeobox 1 is required for retinal neurogenesis and photoreceptor differentiation in embryonic zebrafish, 24-39, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.