ZFIN ID: ZDB-FIG-090331-7
Nelson et al., 2009 - Retinal homeobox 1 is required for retinal neurogenesis and photoreceptor differentiation in embryonic zebrafish. Developmental Biology   328(1):24-39 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 1 Morpholino-mediated depletion of rx1 results in lamination defects and reduced retinal cell differentiation. Embryos were treated at the 1–2 cell stage with a morpholino cocktail containing ATG (translational start site, 100 μM) and GT (splice donor site, 50 μM) directed morpholinos. (A–D) 5 μm sections stained with DAPI obtained at 53 hpf (A, B) and 72 hpf (C, D) from control (A, C) and rx-1-depleted (B, D) embryos, showing inhibition/delay of lamination in treated retinas. (E–H) Sectioned retinas stained for the ganglion cell surface marker, zn-8, evaluated at 53 hpf (E, F) and 72 hpf (G, H) from control (E, G) and rx1-depleted (F, H) retinas, showing patchy staining of the gcl as a consequence of treatment. (I–L) Sections stained for the amacrine and ganglion cell marker HuC/D, obtained at 53 hpf (I, J) and 72 hpf (K, L) from control (I, K) and rx1-depleted (J, L) embryos, showing reduced staining as a consequence of treatment. (M–P) Sections stained with the red/green double cone marker zpr1, from embryos fixed at 53 hpf (M, N) and 72 hpf (O, P) following treatment with control (M, O) and rx1-depleting (N, P) morpholinos, showing reduced cone differentiation in the treated retinas. (Q–T) Sections stained with the rod marker zpr3, from embryos fixed at 53 hpf (Q, R) and 72 hpf (S, T) following treatment with control (Q, S) and rx1-depleting (R, T) morpholinos, showing reduced rod differentiation in the treated retinas. (U–X) Sections stained for the rod bipolar cell marker, PCK (U, V) and the Müller glia cell marker GS (W, X) from control (U, W) and rx1-depleted (V, X) embryos fixed at 72 hpf, showing reduced differentiation of rod bipolar cells and the absence of Müller glia in treated retinas. le = lens; gcl = ganglion cell layer; inl = inner nuclear layer; onl = outer nuclear layer; V = ventral; D = dorsal; dotted lines indicate retinal boundary; white circles depict the location of the lens; scale bar = 50 μm.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-elavl IHC WT control Long-pec retinal ganglion cell layer
IHC Long-pec retinal inner nuclear layer
IHC Protruding-mouth retinal ganglion cell layer
IHC Protruding-mouth retinal inner nuclear layer
IHC WT + MO2-rx1 + MO3-rx1 standard conditions Long-pec retinal ganglion cell layer
IHC Protruding-mouth retinal ganglion cell layer
Ab1-glul IHC WT control Protruding-mouth Muller cell
IHC WT + MO2-rx1 + MO3-rx1 standard conditions Protruding-mouth Not Detected Muller cell
zn-8 IHC WT control Long-pec retinal ganglion cell layer
IHC Protruding-mouth retinal ganglion cell layer
IHC WT + MO2-rx1 + MO3-rx1 standard conditions Long-pec retinal ganglion cell layer
IHC Protruding-mouth retinal ganglion cell layer
zpr-1 IHC WT control Long-pec retinal outer nuclear layer
IHC Protruding-mouth retinal cone cell
IHC WT + MO2-rx1 + MO3-rx1 standard conditions Long-pec retina
IHC Protruding-mouth retina
zpr-3 IHC WT control Long-pec retinal outer nuclear layer
IHC Protruding-mouth retinal rod cell
IHC WT + MO2-rx1 + MO3-rx1 standard conditions Long-pec retina
IHC Protruding-mouth retina
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-rx1 + MO3-rx1 standard conditions Long-pec retina decreased size, abnormal
WT + MO2-rx1 + MO3-rx1 standard conditions Long-pec retina lacks all parts of type Muller cell, abnormal
WT + MO2-rx1 + MO3-rx1 standard conditions Long-pec retina layer formation disrupted, abnormal
WT + MO2-rx1 + MO3-rx1 standard conditions Long-pec retinal bipolar neuron differentiation disrupted, abnormal
WT + MO2-rx1 + MO3-rx1 standard conditions Long-pec retinal cone cell differentiation disrupted, abnormal
WT + MO2-rx1 + MO3-rx1 standard conditions Long-pec retinal rod cell differentiation disrupted, abnormal
WT + MO2-rx1 + MO3-rx1 standard conditions Protruding-mouth retina decreased size, abnormal
WT + MO2-rx1 + MO3-rx1 standard conditions Protruding-mouth retina lacks all parts of type Muller cell, abnormal
WT + MO2-rx1 + MO3-rx1 standard conditions Protruding-mouth retina layer formation delayed, abnormal
WT + MO2-rx1 + MO3-rx1 standard conditions Protruding-mouth retinal bipolar neuron differentiation disrupted, abnormal
WT + MO2-rx1 + MO3-rx1 standard conditions Protruding-mouth retinal cone cell differentiation disrupted, abnormal
WT + MO2-rx1 + MO3-rx1 standard conditions Protruding-mouth retinal outer nuclear layer decreased thickness, abnormal
WT + MO2-rx1 + MO3-rx1 standard conditions Protruding-mouth retinal rod cell differentiation disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 328(1), Nelson, S.M., Park, L., and Stenkamp, D.L., Retinal homeobox 1 is required for retinal neurogenesis and photoreceptor differentiation in embryonic zebrafish, 24-39, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.