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ZFIN ID: ZDB-FIG-090331-8
Nelson et al., 2009 - Retinal homeobox 1 is required for retinal neurogenesis and photoreceptor differentiation in embryonic zebrafish. Developmental Biology   328(1):24-39 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 2 Capped rx1 mRNAs rescue the rx1 depletion phenotype. (A–C) Sectioned embryos processed for indirect immunofluorescence with zpr1 (cone marker), and counterstained with DAPI to reveal retinal lamination. Embryos were injected with 5-mispair control MOs (A), splice site-directed rx1-MOs (B), or splice site-directed rx1-MOs in combination with capped rx1 mRNAs, arrow indicates zpr1+ photoreceptors in ventral retina (C). le = lens; gcl = ganglion cell layer; inl = inner nuclear layer; onl = outer nuclear layer; V = ventral; D = dorsal; scale bar in A (applies to all) = 50 μm.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
zpr-1 IHC WT control Long-pec retinal cone cell
IHC WT + MO2-rx1 + MO3-rx1 standard conditions Long-pec Not Detected retinal cone cell
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-rx1 + MO3-rx1 standard conditions Long-pec retina layer formation disrupted, abnormal
Long-pec retinal cone cell differentiation disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 328(1), Nelson, S.M., Park, L., and Stenkamp, D.L., Retinal homeobox 1 is required for retinal neurogenesis and photoreceptor differentiation in embryonic zebrafish, 24-39, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.