ZFIN ID: ZDB-FIG-090331-16
Nelson et al., 2009 - Retinal homeobox 1 is required for retinal neurogenesis and photoreceptor differentiation in embryonic zebrafish. Developmental Biology   328(1):24-39 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 11 Embryos subjected to temporally-selective (at 44.5 hpf) depletion of rx1 and rx2 show a significant reduction in photoreceptor differentiation. (A–B) DAPI-stained retinal cryosections obtained from control (A) and rx1/rx2-depleted (B) embryos fixed at 72 hpf; onl appears normal in both cases (arrow in B). (C–F) Retinal cryosections obtained from control (C, E) and rx1/rx2-depleted (D, F) embryos fixed at 72 hpf and hybridized with cRNA probes corresponding to NeuroD (C, D) and crx (E, F), showing no effect of retinal homeobox depletion on expression of these transcription factors. (G–J) Retinal cryosections obtained from control (G, I) and rx1/rx2-depleted (H,J) embryos fixed at 72 hpf and stained with the zpr1 (cones; G, H) and zpr3 (rods; I, J) antibodies, showing elimination of markers of photoreceptor terminal differentiation as a consequence of temporally-selective retinal homeobox knockdown. (K–M) Sections bisecting the lens were scored for the absence (‘none’) or presence of fewer than ten (‘few’) or more than ten (‘many’) zpr-1 or zpr3-stained photoreceptors. Relative numbers of sections displaying the indicate score are shown following temporally-selective depletion of rx1 (K), rx2 (L), and rx1 and rx2 (M). Knockdown of both rx1 and rx2 is more effective at reducing photoreceptor differentiation. le = lens, gcl = ganglion cell layer, inl = inner nuclear layer, onl = outer nuclear layer, scale bar in A = 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
crx WT control Protruding-mouth retina ISH
WT + MO2-rx1 + MO2-rx2 + MO3-rx1 + MO3-rx2 standard conditions Protruding-mouth retina ISH
neurod1 WT control Protruding-mouth retina ISH
WT + MO2-rx1 + MO2-rx2 + MO3-rx1 + MO3-rx2 standard conditions Protruding-mouth retina ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
zpr-1 IHC WT control Protruding-mouth retinal cone cell
IHC WT + MO2-rx1 + MO2-rx2 + MO3-rx1 + MO3-rx2 standard conditions Protruding-mouth Not Detected retina
zpr-3 IHC WT control Protruding-mouth retinal rod cell
IHC WT + MO2-rx1 + MO2-rx2 + MO3-rx1 + MO3-rx2 standard conditions Protruding-mouth Not Detected retina
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-rx1 + MO2-rx2 + MO3-rx1 + MO3-rx2 standard conditions Protruding-mouth eye photoreceptor cell differentiation disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 328(1), Nelson, S.M., Park, L., and Stenkamp, D.L., Retinal homeobox 1 is required for retinal neurogenesis and photoreceptor differentiation in embryonic zebrafish, 24-39, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.