ZFIN ID: ZDB-FIG-090331-9
Nelson et al., 2009 - Retinal homeobox 1 is required for retinal neurogenesis and photoreceptor differentiation in embryonic zebrafish. Developmental Biology   328(1):24-39 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: 10-13 somites

Fig. 3 Rx2 but not rx1 depletion causes a reduction in pax6 expression in optic primordia. (A–C) Embryos injected with MOs at the 1–2 cell stage and hybridized with a pax6 probe at 15 hpf. (A) 5-mispair control treated embryo (n = 48) showing the three regions of pax6 expression (arrowhead indicates anterior midline, arrow indicates eye anlage, asterisk indicates posterior midline). (B) rx1-depleted embryo showing similar pattern of expression of pax6 (n = 33). (C) rx2-depleted embryo (n = 24) showing a reduction in pax6 expression in the eye anlage and anterior midline. (D–F) Histograms showing the relative expression levels of pax6 in the eye anlage, anterior midline, and posterior midline, respectively, in 5-mispair control, rx1 and rx2 morphants. Scale bar in A (applies to all) = 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
pax6a WT control 10-13 somites optic vesicle ISH
WT + MO2-rx1 + MO3-rx1 standard conditions 10-13 somites optic vesicle ISH
WT + MO2-rx2 + MO3-rx2 standard conditions 10-13 somites optic vesicle ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 328(1), Nelson, S.M., Park, L., and Stenkamp, D.L., Retinal homeobox 1 is required for retinal neurogenesis and photoreceptor differentiation in embryonic zebrafish, 24-39, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.