ZFIN ID: ZDB-FIG-090331-11
Nelson et al., 2009 - Retinal homeobox 1 is required for retinal neurogenesis and photoreceptor differentiation in embryonic zebrafish. Developmental Biology   328(1):24-39 Full text @ Dev. Biol.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Protruding-mouth

Fig. 5 Rx1 morphants but not rx2 morphants exhibit significant changes in retinal progenitor proliferation. (A–H) Retinal cryosections stained with anti-PH3, a marker for cells in M-phase, from embryos fixed at 25 hpf (A, B), 34 hpf (C, D), 53 hpf (E, F), and 72 hpf (G, H), injected with control MOs (A, C, E, G) or rx1-depleting MOs (B, D, F, H). Staining is markedly reduced in the rx1 morphants at the early times, but increased at the later times; arrow in D indicates disorganized mitotic cells that were occasionally observed in the morphants. I. Average numbers of PH3-positive cells as a function of treatment and time of assessment. Significant differences were evident following rx1 depletion for all times evaluated (***p < 0.001) but rx2 depletion caused no changes. Scale bar = 50 μm; error bars indicate ± 1 SD.

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-rx1 + MO3-rx1 standard conditions Prim-5 - Protruding-mouth cell population proliferation disrupted, abnormal
Prim-5 - Protruding-mouth neural retina development disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 328(1), Nelson, S.M., Park, L., and Stenkamp, D.L., Retinal homeobox 1 is required for retinal neurogenesis and photoreceptor differentiation in embryonic zebrafish, 24-39, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.