Publication	Data		Fish	Anatomy
Barske et al., 2018	Fig. 3		edn1tf216b/tf216b (TU)	Meckel's cartilage decreased size, abnormal Meckel's cartilage fused with palatoquadrate arch, abnormal
	Fig. 5		edn1tf216b/tf216b (TU)	mandibular arch skeleton nr2f1a expression increased amount, abnormal mandibular arch skeleton nr2f1b expression increased amount, abnormal mandibular arch skeleton nr2f2 expression increased amount, abnormal mandibular arch skeleton nr2f5 expression increased amount, abnormal pharyngeal arch 1 nr2f1a expression increased distribution, abnormal pharyngeal arch 1 nr2f1b expression increased distribution, abnormal pharyngeal arch 1 nr2f2 expression increased distribution, abnormal pharyngeal arch 1 nr2f5 expression increased distribution, abnormal pharyngeal arch 2 nr2f1a expression increased distribution, abnormal pharyngeal arch 2 nr2f1b expression increased distribution, abnormal pharyngeal arch 2 nr2f2 expression increased distribution, abnormal pharyngeal arch 2 nr2f5 expression increased distribution, abnormal
	Fig. 6		edn1tf216b/tf216b (TU)	Meckel's cartilage absent, abnormal pharyngeal arch 1 dlx5a expression decreased amount, abnormal pharyngeal arch 1 dlx6a expression decreased amount, abnormal pharyngeal arch 1 hand2 expression decreased amount, abnormal pharyngeal arch 1 jag1b expression increased distribution, abnormal pharyngeal arch 1 prrx1b expression increased distribution, abnormal pharyngeal arch 2 dlx5a expression decreased amount, abnormal pharyngeal arch 2 dlx6a expression decreased amount, abnormal pharyngeal arch 2 hand2 expression decreased amount, abnormal pharyngeal arch 2 jag1b expression increased distribution, abnormal pharyngeal arch 2 prrx1b expression increased distribution, abnormal quadrate-anguloarticular joint absent, abnormal ventral mandibular arch decreased size, abnormal
Xu et al., 2018	Fig. 1		edn1tf216b/tf216b	pharyngeal arch 1 foxc1a expression absent, abnormal pharyngeal arch 1 foxd2 expression increased distribution, abnormal pharyngeal arch 1 foxf1 expression decreased amount, abnormal pharyngeal arch 2 foxc1a expression absent, abnormal pharyngeal arch 2 foxd2 expression increased distribution, abnormal pharyngeal arch 2 foxf1 expression spatial pattern, abnormal
	Fig. S2		edn1tf216b/tf216b	pharyngeal arch 1 foxc1b expression decreased amount, abnormal pharyngeal arch 1 foxd1 expression decreased amount, abnormal pharyngeal arch 1 foxd1 expression decreased distribution, abnormal pharyngeal arch 2 foxc1b expression decreased amount, abnormal pharyngeal arch 2 foxd1 expression decreased amount, abnormal pharyngeal arch 2 foxd1 expression decreased distribution, abnormal
Askary et al., 2017	Fig. 4		edn1tf216b/tf216b; el10Tg; y1Tg	pharyngeal arch 1 medial region ccn2b expression decreased amount, abnormal pharyngeal arch 1 medial region cdh11 expression spatial pattern, abnormal pharyngeal arch 1 medial region fsta expression decreased amount, abnormal pharyngeal arch 1 medial region pou3f3a expression increased distribution, abnormal pharyngeal arch 1 medial region pou3f3b expression increased distribution, abnormal pharyngeal arch 1 ventral region fgfbp2a expression absent, abnormal pharyngeal arch 1 ventral region pou3f3a expression increased distribution, abnormal pharyngeal arch 1 ventral region pou3f3b expression increased distribution, abnormal pharyngeal arch 1 ventral region sema3bl expression absent, abnormal pharyngeal arch 1 ventral region shox expression absent, abnormal pharyngeal arch 1 ventral region stmn1a expression absent, abnormal pharyngeal arch 1 ventral-most region pitx1 expression absent, abnormal pharyngeal arch 2 medial region ccn2b expression decreased amount, abnormal pharyngeal arch 2 medial region cdh11 expression spatial pattern, abnormal pharyngeal arch 2 medial region fsta expression decreased amount, abnormal pharyngeal arch 2 medial region pou3f3a expression increased distribution, abnormal pharyngeal arch 2 medial region pou3f3b expression increased distribution, abnormal pharyngeal arch 2 ventral region fgfbp2a expression absent, abnormal pharyngeal arch 2 ventral region pou3f3a expression increased distribution, abnormal pharyngeal arch 2 ventral region pou3f3b expression increased distribution, abnormal pharyngeal arch 2 ventral region shox expression absent, abnormal pharyngeal arch 2 ventral region stmn1a expression absent, abnormal ventral hyoid arch fsta expression mislocalised, abnormal
	Fig. S4		edn1tf216b/tf216b	opercle increased variability of size, abnormal
Iklé et al., 2017	Fig. 2		WT + MO1-edn1 + MO4-tp53	ceratobranchial 1 cartilage absent, abnormal ceratobranchial 2 cartilage absent, abnormal ceratobranchial 3 cartilage absent, abnormal ceratobranchial 4 cartilage absent, abnormal ceratohyal cartilage curved ventral, abnormal ceratohyal cartilage hypoplastic, abnormal Meckel's cartilage curved ventral, abnormal Meckel's cartilage fused with palatoquadrate cartilage, abnormal Meckel's cartilage hypoplastic, abnormal pharyngeal arch 1 dlx5a expression absent, abnormal pharyngeal arch 2 dlx5a expression absent, abnormal
Barske et al., 2016	Fig. 3		edn1tf216b/tf216b	pharyngeal arch 2 barx1 expression decreased amount, abnormal
	Fig. 6		edn1tf216b/tf216b	pharyngeal arch neural crest cell prrx1a expression increased amount, abnormal pharyngeal arch neural crest cell prrx1b expression increased amount, abnormal pharyngeal arch ventral region prrx1a expression increased amount, abnormal pharyngeal arch ventral region prrx1b expression increased amount, abnormal
	Fig. 8		edn1tf216b/tf216b	pharyngeal arch 2 ventral region barx1 expression decreased amount, abnormal pharyngeal arch 2 ventral region prrx1b expression increased amount, abnormal
	Fig. 9		edn1tf216b/tf216b	pharyngeal arch 1 ventral region decreased amount, abnormal pharyngeal arch 1 ventral region decreased size, abnormal pharyngeal arch 2 ventral region decreased amount, abnormal pharyngeal arch 2 ventral region decreased size, abnormal pharyngeal arch cartilage ventral region decreased amount, abnormal pharyngeal arch cartilage ventral region morphology, abnormal pharyngeal arch ventral region barx1 expression decreased amount, abnormal pharyngeal arch ventral region dlx5a expression decreased amount, abnormal
Askary et al., 2015	Fig. 1		edn1tf216b/tf216b	pharyngeal arch 2 joint irx5a expression absent, abnormal pharyngeal arch 2 joint irx7 expression absent, abnormal
Kamel et al., 2013	Fig. 7		edn1tf216b/tf216b	hindbrain fzd7a expression absent, abnormal pharyngeal arch frzb expression absent, abnormal pharyngeal arch fzd7a expression decreased amount, abnormal pharyngeal arch nkx3-2 expression absent, abnormal pharyngeal arch wnt9a expression absent, abnormal
Sasaki et al., 2013	Fig. 1		edn1tf216b/tf216b (AB)	basihyal cartilage deformed, abnormal ceratohyal cartilage deformed, abnormal hyosymplectic cartilage aplastic, abnormal interhyal cartilage absent, abnormal interhyal-epihyal joint absent, abnormal Meckel's cartilage decreased size, abnormal retroarticular absent, abnormal
	Fig. 2		edn1tf216b/tf216b; y1Tg (AB)	pharyngeal arch 1 decreased length, abnormal
	Fig. 3		edn1tf216b/tf216b; y1Tg (AB)	pharyngeal arch 1 decreased length, abnormal pharyngeal arch 1 mesenchyme decreased area, abnormal
Choudhry et al., 2011	Fig. 2		edn1tf216b/tf216b	Meckel's cartilage bent, abnormal Meckel's cartilage fused with palatoquadrate cartilage, abnormal Meckel's cartilage protruding, abnormal palatoquadrate cartilage fused with hyosymplectic cartilage, abnormal palatoquadrate cartilage increased length, abnormal symplectic absent, abnormal
Zuniga et al., 2011	Fig. 7		edn1tf216b/tf216b	pharyngeal arch 1 malformed, abnormal pharyngeal arch 2 malformed, abnormal
Wada et al., 2010	Fig. 5		WT + MO1-edn1	anterior lateral line neuromast increased amount, abnormal anterior lateral line neuromast mislocalised, abnormal opercle increased width, abnormal opercle shape, abnormal opercular lateral line neuromast mislocalised dorsally, abnormal
Zuniga et al., 2010	Fig. 6		edn1tf216b/tf216b	branchiostegal ray decreased size, abnormal ceratohyal cartilage absent, abnormal ceratohyal cartilage decreased size, abnormal Meckel's cartilage absent, abnormal Meckel's cartilage decreased size, abnormal opercle increased variability of size, abnormal symplectic absent, abnormal symplectic decreased size, abnormal
Nair et al., 2007	Fig. 3		edn1tf216b/tf216b	ceratohyal cartilage aplastic, abnormal Meckel's cartilage decreased size, abnormal ventral mandibular arch aplastic, abnormal
Milan et al., 2006	Fig. 5		WT + MO1-edn1	pharyngeal system development disrupted, abnormal
Nissen et al., 2006	Fig. 4		WT + MO1-edn1	ceratohyal cartilage aplastic, abnormal Meckel's cartilage aplastic, abnormal
Phenotype Annotation (1994-2006)	Fig. for (tf216b)		edn1tf216b/tf216b	mouth quality, abnormal pharyngeal arch 3-7 skeleton quality, abnormal splanchnocranium quality, abnormal whole organism lethal (sensu genetics), abnormal
Walker et al., 2006	Fig. 3		WT + MO1-edn1 ,   edn1tf216b/tf216b	ceratohyal cartilage aplastic, abnormal interhyal-epihyal joint absent, abnormal mandibular arch skeleton joint absent, abnormal Meckel's cartilage decreased size, abnormal palatoquadrate cartilage decreased size, abnormal
Kimmel et al., 2003	Fig. 2		edn1tf216b/tf216b	ceratobranchial 5 bone decreased length, abnormal ceratobranchial 5 bone malformed, abnormal cleithrum decreased length, abnormal cleithrum malformed, abnormal dentary malformed, abnormal maxilla malformed, abnormal opercle cartilaginous joint distended, abnormal ossification disrupted, abnormal
	Fig. 3		edn1tf216b/tf216b	dentary curved caudal, abnormal dentary fused with maxilla, abnormal dentary malformed, abnormal maxilla curved caudal, abnormal maxilla malformed, abnormal ventral mandibular arch orientation eye, abnormal
	Fig. 4		edn1tf216b/tf216b	opercle cartilaginous joint distended, abnormal opercle cartilaginous joint increased thickness, abnormal opercle increased size, abnormal
	Fig. 6		WT + MO1-edn1	opercle size, abnormal pharyngeal arch 2 skeleton decreased size, abnormal
	Fig. 7		WT + MO1-edn1	infraorbital lateral line neuromast decreased amount, abnormal middle lateral line neuromast decreased amount, abnormal opercle decreased size, abnormal opercular lateral line neuromast absent, abnormal otic lateral line neuromast decreased amount, abnormal
	Fig. 8		WT + MO1-edn1	branchiostegal ray falciform, abnormal branchiostegal ray malformed, abnormal branchiostegal ray position, abnormal ceratohyal-branchiostegal ray joint morphology, abnormal opercle increased size, abnormal
	Fig. Table 1		edn1tf216b/tf216b	branchiostegal ray decreased size, abnormal opercle size, abnormal
	Fig. Table 2		WT + MO1-edn1	opercle decreased size, abnormal opercular lateral line neuromast decreased amount, abnormal
Miller et al., 2003	Fig. 3		edn1tf216b/tf216b	embryonic viscerocranium morphogenesis disrupted, abnormal pharyngeal arch 1 joint physical object quality, abnormal
	Fig. 8		edn1tf216b/tf216b	embryonic viscerocranium morphogenesis disrupted, abnormal mandibular muscle wholly dorsalized, abnormal
Miller et al., 2000	Fig. 1		edn1tf216b/tf216b	embryonic viscerocranium morphogenesis decreased process quality, abnormal hyosymplectic cartilage fused with ceratohyal cartilage, abnormal palatoquadrate cartilage fused with Meckel's cartilage, abnormal
	Fig. 5		edn1tf216b/tf216b	embryonic viscerocranium morphogenesis decreased process quality, abnormal pharyngeal arch 1 mesenchymal cell physical object quality, abnormal pharyngeal arch 1 ventral region physical object quality, abnormal pharyngeal arch 2 mesenchymal cell physical object quality, abnormal pharyngeal arch 2 ventral region physical object quality, abnormal pharyngeal arch 3 mesenchymal cell physical object quality, abnormal pharyngeal arch 3 ventral region physical object quality, abnormal pharyngeal arch 4 mesenchymal cell physical object quality, abnormal pharyngeal arch 4 ventral region physical object quality, abnormal
	Fig. 6		edn1tf216b/tf216b	embryonic viscerocranium morphogenesis decreased process quality, abnormal pharyngeal arch 1 mesenchymal cell physical object quality, abnormal pharyngeal arch 1 ventral region physical object quality, abnormal pharyngeal arch 2 mesenchymal cell physical object quality, abnormal pharyngeal arch 2 ventral region physical object quality, abnormal pharyngeal arch 3 mesenchymal cell physical object quality, abnormal pharyngeal arch 3 ventral region physical object quality, abnormal pharyngeal arch 4 mesenchymal cell physical object quality, abnormal pharyngeal arch 4 ventral region physical object quality, abnormal
	Fig. 7		edn1tf216b/tf216b	branchiomeric skeletal muscle development decreased process quality, abnormal head lacks all parts of type ventral intermandibularis anterior, abnormal head lacks all parts of type ventral intermandibularis posterior, abnormal hyohyoideus decreased length, abnormal interhyoideus decreased length, abnormal interhyoideus physical object quality, abnormal pharyngeal pouch 1 physical object quality, abnormal stomodeum mislocalised ventrally, abnormal ventral intermandibularis anterior physical object quality, abnormal ventral intermandibularis posterior physical object quality, abnormal
Kimmel et al., 1998	Fig. 10		edn1tf216b/tf216b	cartilage morphogenesis disrupted, abnormal ceratohyal cartilage decreased size, abnormal ceratohyal cartilage morphology, abnormal interhyal-epihyal joint absent, abnormal Meckel's cartilage decreased size, abnormal Meckel's cartilage morphology, abnormal opercle aplastic, abnormal pharyngeal arch 1 position, abnormal pharyngeal arch 2 position, abnormal
	Fig. 9		edn1tf216b/tf216b	cartilage morphogenesis disrupted, abnormal ceratohyal cartilage decreased size, abnormal ceratohyal cartilage inverted, abnormal ceratohyal cartilage structure, abnormal interhyal-epihyal joint absent, abnormal ventral hyoid arch morphology, abnormal
Piotrowski et al., 1996	Fig. 2		edn1tf216b/tf216b	ceratohyal cartilage decreased size, abnormal hyosymplectic cartilage increased width, abnormal Meckel's cartilage aplastic, abnormal Meckel's cartilage decreased size, abnormal Meckel's cartilage mislocalised posteriorly, abnormal mouth concave 3-D shape, abnormal palatoquadrate cartilage decreased size, abnormal pharyngeal arch 3 skeleton decreased length, abnormal pharyngeal arch 4 skeleton decreased length, abnormal ventral mandibular arch decreased size, abnormal