PUBLICATION
Essential Role of Nr2f Nuclear Receptors in Patterning the Vertebrate Upper Jaw
- Authors
- Barske, L., Rataud, P., Behizad, K., Del Rio, L., Cox, S.G., Crump, J.G.
- ID
- ZDB-PUB-180124-2
- Date
- 2018
- Source
- Developmental Cell 44(3): 337-347.e5 (Journal)
- Registered Authors
- Barske, Lindsey, Crump, Gage DeKoeyer
- Keywords
- COUP-TF, Endothelin1, Nr2f1, Nr2f2, Nr2f5, bone, cartilage, jaw, nuclear receptor, pharyngeal arches
- Datasets
- GEO:GSE101719
- MeSH Terms
-
- Animals
- Body Patterning/physiology*
- Chondrogenesis/physiology
- DNA-Binding Proteins/genetics
- DNA-Binding Proteins/metabolism*
- Endothelin-1/genetics
- Endothelin-1/metabolism*
- Gene Expression Regulation, Developmental
- Jaw/embryology*
- Jaw/physiology
- Maxilla/embryology*
- Maxilla/physiology
- Mutation
- Odontogenesis/physiology
- Signal Transduction
- Transcription Factors/genetics
- Transcription Factors/metabolism*
- Zebrafish/embryology*
- Zebrafish/physiology
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 29358039 Full text @ Dev. Cell
Citation
Barske, L., Rataud, P., Behizad, K., Del Rio, L., Cox, S.G., Crump, J.G. (2018) Essential Role of Nr2f Nuclear Receptors in Patterning the Vertebrate Upper Jaw. Developmental Cell. 44(3):337-347.e5.
Abstract
The jaw is central to the extensive variety of feeding and predatory behaviors across vertebrates. The bones of the lower but not upper jaw form around an early-developing cartilage template. Whereas Endothelin1 patterns the lower jaw, the factors that specify upper-jaw morphology remain elusive. Here, we identify Nuclear Receptor 2f genes (Nr2fs) as enriched in and required for upper-jaw formation in zebrafish. Combinatorial loss of Nr2fs transforms maxillary components of the upper jaw into lower-jaw-like structures. Conversely, nr2f5 misexpression disrupts lower-jaw development. Genome-wide analyses reveal that Nr2fs repress mandibular gene expression and early chondrogenesis in maxillary precursors. Rescue of lower-jaw defects in endothelin1 mutants by reducing Nr2f dosage further demonstrates that Nr2f expression must be suppressed for normal lower-jaw development. We propose that Nr2fs shape the upper jaw by protecting maxillary progenitors from early chondrogenesis, thus preserving cells for later osteogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping