ZFIN ID: ZDB-FIG-101213-8
Kimmel et al., 2003 - Endothelin 1-mediated regulation of pharyngeal bone development in zebrafish. Development (Cambridge, England)   130(7):1339-1351 Full text @ Development
Knockdown Reagent:
Observed In:
Stage: Day 6

Fig. 7 Correlated expressivities of opercle and lateral line neuromast phenotypes in edn1-MO-injected larvae (C,D), compared with an uninjected larva (A,B). Left-side views of live larvae, with dorsal to the top and anterior to the left at 6-days post fertilization. (B-D) Confocal z-series projections. (A) A single optical section from the image stack in B combining DIC with fluorescence for orientation. The bones are labeled with Calcein (green), and the neuromasts with DASPEI (orange-red). Neuromasts were identified (Raible and Kruse, 2000) and are labeled with upper case letters; other structures are labeled in lower case. (B) The normal neuromast distribution in the uninjected control. (C) An edn1-MO-injected larva expressing the opercle-gain phenotype, the branchiostegal ray (bsr) is also malformed. The complete set of neuromasts is present, however IO3 is displaced ventrally and M1 is displaced posteriorly. (D) An edn1-MO-injected larva expressing the opercle-loss phenotype. Both the opercle and branchiostegal ray are missing, and the mandibular dermal bones (max/den) resemble the condition in Fig. 3E. The neuromast phenotype in this particular larva is severe; losses include M1, IO2, O2 and the two opercular neuromasts OP1 and OP2. Scale bar: 100 μm. MO, morpholino.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-edn1 standard conditions Day 6 infraorbital lateral line neuromast decreased amount, abnormal
Day 6 middle lateral line neuromast decreased amount, abnormal
Day 6 opercle decreased size, abnormal
Day 6 opercular lateral line neuromast absent, abnormal
Day 6 otic lateral line neuromast decreased amount, abnormal
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development