ZFIN ID: ZDB-FIG-180608-105
Barske et al., 2018 - Essential Role of Nr2f Nuclear Receptors in Patterning the Vertebrate Upper Jaw. Developmental Cell   44(3):337-347.e5 Full text @ Dev. Cell
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Day 5

Fig. 3

nr2f5 Misexpression Reduces and Transforms the Lower Jaw

(A–G) Alcian blue and alizarin red staining of dissected facial skeletons shown from the ventral perspective. The upper jaw (Ptp, green in A′–C′) remains, but the lower jaw (M, magenta in A′) is reduced and transformed (black arrows) in sox10:Gal4VP16; UAS:Nr2f5el662 transgenic fish (B), similar to edn1 mutants (C). Putative transformations are schematized in (A′–C′). Late-onset nr2f5 misexpression in chondrocytes (col2a1a:Gal4VP16) did not affect the facial skeleton (D). Misexpression in post-migratory mandibular NCCs (hand2:Gal4VP16) reduced the distal tips of Meckel's (black arrow, E). Heat-shock-induced embryo-wide misexpression (hsp70l:Gal4) reduced the lower jaw (black arrow) when applied at 15–17 (F) but not 19–21 hpf (G).

(H) Approximate timing of Nr2f5 protein overexpression (OE) in the different driver lines, with the approximate window of sufficiency indicated in magenta.

Scale bar, 50 μm. Also see Figure S3 for additional analyses of the sox10:Gal4VP16; UAS:Nr2f5 phenotype.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
b1228Tg ; el663Tg(TU) standard conditions Day 5 Meckel's cartilage distal region decreased size, abnormal
edn1tf216b/tf216b(TU) standard conditions Day 5 Meckel's cartilage decreased size, abnormal
Day 5 Meckel's cartilage fused with palatoquadrate arch, abnormal
el662Tg ; el159Tg(TU) standard conditions Day 5 Meckel's cartilage decreased size, abnormal
Day 5 Meckel's cartilage fused with palatoquadrate arch, abnormal
el662Tg ; el647Tg(TU) standard conditions Day 5 mandibular arch skeleton morphology, normal
kca4Tg ; el662Tg(TU) heat shock Day 5 ventral mandibular arch decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 44(3), Barske, L., Rataud, P., Behizad, K., Del Rio, L., Cox, S.G., Crump, J.G., Essential Role of Nr2f Nuclear Receptors in Patterning the Vertebrate Upper Jaw, 337-347.e5, Copyright (2018) with permission from Elsevier. Full text @ Dev. Cell