PUBLICATION

Notch1b and neuregulin are required for specification of central cardiac conduction tissue

Authors
Milan, D.J., Giokas, A.C., Serluca, F.C., Peterson, R.T., and MacRae, C.A.
ID
ZDB-PUB-060227-6
Date
2006
Source
Development (Cambridge, England)   133(6): 1125-1132 (Journal)
Registered Authors
MacRae, Calum A., Milan, David J., Peterson, Randall, Serluca, Fabrizio
Keywords
Notch, Neuregulin, Cardiac conduction system, Development, Zebrafish
MeSH Terms
  • Animals
  • Endothelin-1/genetics
  • Endothelin-1/metabolism
  • Gene Expression Regulation, Developmental
  • Heart/embryology*
  • Myocardium/metabolism*
  • Neuregulins/genetics
  • Neuregulins/metabolism*
  • Organ Specificity
  • Receptor, Notch1/genetics
  • Receptor, Notch1/metabolism*
  • Renal Circulation
  • Signal Transduction
  • Time Factors
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/metabolism
PubMed
16481353 Full text @ Development
Abstract
Normal heart function is critically dependent on the timing and coordination provided by a complex network of specialized cells: the cardiac conduction system. We have employed functional assays in zebrafish to explore early steps in the patterning of the conduction system that previously have been inaccessible. We demonstrate that a ring of atrioventricular conduction tissue develops at 40 hours post-fertilization in the zebrafish heart. Analysis of the mutant cloche reveals a requirement for endocardial signals in the formation of this tissue. The differentiation of these specialized cells, unlike that of adjacent endocardial cushions and valves, is not dependent on blood flow or cardiac contraction. Finally, both neuregulin and notch1b are necessary for the development of atrioventricular conduction tissue. These results are the first demonstration of the endocardial signals required for patterning central 'slow' conduction tissue, and they reveal the operation of distinct local endocardial-myocardial interactions within the developing heart tube.
Genes / Markers
Figures
Figure Gallery
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes