Regulation of foxc1b and foxd1 arch expression
Expression of foxc1b (A) and foxd1 (B) in green, relative to dlx2a+ CNCs (red) of the first two arches. Similar to foxc1a in Figure 1, foxc1b and foxd1 expression is expanded by Shha misexpression, inhibited by Bmp4 and Fgf3 misexpression, and reduced in edn1 but not jag1b mutants. Embryos doubly transgenic for hsp70l:Gal4 and UAS:shha, UAS:bmp4, or UAS:fgf3 were subjected to a heatshock from 20-24 hpf to induce ligand expression throughout embryos. Numbers indicate proportion of animals showing the displayed patterns. Scale bars = 25 μm.