ZFIN ID: ZDB-PUB-180520-1
Fox proteins are modular competency factors for facial cartilage and tooth specification
Xu, P., Balczerski, B., Ciozda, A., Louie, K., Oralova, V., Huysseune, A., Crump, J.G.
Date: 2018
Source: Development (Cambridge, England)   145(12): (Journal)
Registered Authors: Balczerski, Bartosz, Crump, Gage DeKoeyer, Huysseune, Ann
Keywords: Bone, Cartilage, Craniofacial, Forkhead, Foxc1, Foxf1, Foxf2, Pharyngeal arches, Sox9, Teeth, Zebrafish
MeSH Terms:
  • Animals
  • Body Patterning*/genetics
  • Bone and Bones/metabolism
  • Branchial Region/metabolism
  • Cartilage/embryology*
  • Cartilage/metabolism*
  • Cell Proliferation/genetics
  • Cell Survival/genetics
  • Chondrogenesis/genetics
  • Face
  • Forkhead Transcription Factors/genetics
  • Forkhead Transcription Factors/metabolism*
  • Gene Expression Regulation, Developmental
  • Mutation/genetics
  • Neural Crest/cytology
  • Signal Transduction
  • Skull/cytology
  • Tooth/embryology*
  • Tooth/metabolism*
  • Zebrafish/embryology
  • Zebrafish Proteins/metabolism
PubMed: 29777011 Full text @ Development
Facial form depends on the precise positioning of cartilage, bone, and tooth fields in the embryonic pharyngeal arches. How complex signaling information is integrated to specify these cell types remains a mystery. We find that modular expression of Forkhead-domain transcription factors (Fox) in the zebrafish face arises through integration of Hh, Fgf, Bmp, Edn1, and Jagged-Notch pathways. Whereas loss of C-class Fox proteins results in reduced upper facial cartilages, loss of F-class Fox proteins results in distal jaw truncations and absent midline cartilages and teeth. We show that Fox proteins are required for Sox9a to promote chondrogenic gene expression. Fox proteins are sufficient in neural crest-derived cells for cartilage development, and neural crest-specific misexpression of Fox proteins expands the cartilage domain while inhibiting bone. These results support a modular role for Fox proteins in establishing the competency of progenitors to form cartilage and teeth in the face.