ZFIN ID: ZDB-FIG-060809-13
Nissen et al., 2006 - A zebrafish screen for craniofacial mutants identifies wdr68 as a highly conserved gene required for Endothelin-1 expression. BMC Developmental Biology   6:28 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Day 4

Fig. 4 wdr68 activity is required for all 1st arch cartilage formation. A), C), E), G) Lateral views of Alcian blue stained animals at 4dpf. B), D), F), H) Ventral views of Alcian blue stained animals at 4dpf. In panels D, F, and H the posterior arches were removed to enhance clarity for observing the anterior arch defects. A), B) Wild type sibling. C), D) Representative 'strong' phenotype dys/dys animal similar to that shown flatmounted in figure 1 panel D with incomplete loss of M and PQ cartilages. The wdr68MO when injected into wild type animals yields a phenotype very similar to that shown here in panels C and D. E), F) Injecting dys/dys animals with antisense wdr68MO causes complete loss of the PQ, M and CH but does not substantially reduce the HM region. G), H) Representative edn1MO knockdown animal lacking the M and CH but retaining the PQ and HM regions.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-dcaf7 standard conditions Day 4 Meckel's cartilage decreased size, abnormal
Day 4 palatoquadrate cartilage decreased size, abnormal
WT + MO1-edn1 standard conditions Day 4 ceratohyal cartilage aplastic, abnormal
Day 4 Meckel's cartilage aplastic, abnormal
dcaf7hi3812Tg/hi3812Tg standard conditions Day 4 Meckel's cartilage decreased size, abnormal
Day 4 palatoquadrate cartilage decreased size, abnormal
dcaf7hi3812Tg/hi3812Tg + MO1-dcaf7 standard conditions Day 4 ceratohyal cartilage aplastic, abnormal
Day 4 Meckel's cartilage aplastic, abnormal
Day 4 metapterygoid morphology, abnormal
Day 4 palatoquadrate cartilage aplastic, abnormal
Day 4 symplectic decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.