ZFIN ID: ZDB-FIG-180608-107
Barske et al., 2018 - Essential Role of Nr2f Nuclear Receptors in Patterning the Vertebrate Upper Jaw. Developmental Cell   44(3):337-347.e5 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: 20-25 somites to Prim-15
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Prim-15

Fig. 5

Edn1 Inhibits Nr2f Expression in the Mandibular Prominence

(A) edn1 is expressed comparably in the ventral ectoderm of wild-type controls and mutants at 20 hpf (arrowheads) and 30 hpf (magenta).

(B) Expression of the Edn1 receptors is reduced (ednraa) or largely unaffected (ednrab) in Nr2f mutants. Arch NCCs are labeled with dlx2a (green), and nr2f1b genotypes are indicated.

(C) In edn1 mutants, expression of all four Nr2f genes (magenta) ectopically increases to varying degrees in the mandibular domain (white arrows). Misexpression of edn1 induced by a heat shockof hsp70l:Gal4; UAS:Edn1 embryos from 20 to 24 hpf reduced Nr2f expression throughout the arches. Dashed lines reflect arch boundaries. Images are maximum-intensity projections. Merged images with dlx2a arch NCC staining are presented in Figure S5.

Scale bars, (A, top) 100 μm, (A, bottom, B, C) 20 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dlx2a TU standard conditions Prim-15 pharyngeal arch 1 neural crest cell ISH
Prim-15 pharyngeal arch 2 neural crest cell ISH
nr2f5el611/el611 ; nr2f1bel523/+ ; nr2f2el506/el506(TU) standard conditions Prim-15 pharyngeal arch 1 neural crest cell ISH
Prim-15 pharyngeal arch 2 neural crest cell ISH
nr2f5el611/el611 ; nr2f2el506/el506(TU) standard conditions Prim-15 pharyngeal arch 1 neural crest cell ISH
Prim-15 pharyngeal arch 2 neural crest cell ISH
edn1 TU standard conditions 20-25 somites non neural ectoderm ISH
Prim-15 pharyngeal arch 1 ISH
Prim-15 pharyngeal arch 2 ISH
nr2f5el611/el611 ; nr2f1bel523/+ ; nr2f2el506/el506(TU) standard conditions Prim-15 pharyngeal arch 1 ISH
Prim-15 pharyngeal arch 2 ISH
nr2f5el611/el611 ; nr2f2el506/el506 ; nr2f1bel523/el523(TU) standard conditions 20-25 somites non neural ectoderm ISH
ednraa TU standard conditions Prim-15 pharyngeal arch 1 ISH
Prim-15 pharyngeal arch 2 ISH
nr2f5el611/el611 ; nr2f1bel523/+ ; nr2f2el506/el506(TU) standard conditions Prim-15 pharyngeal arch 1 ISH
Prim-15 pharyngeal arch 2 ISH
ednrab TU standard conditions Prim-15 pharyngeal arch 1 ISH
Prim-15 pharyngeal arch 2 ISH
nr2f5el611/el611 ; nr2f2el506/el506(TU) standard conditions Prim-15 pharyngeal arch 1 ISH
Prim-15 pharyngeal arch 2 ISH
nr2f1a TU standard conditions Prim-15 pharyngeal arch 1 ISH
Prim-15 pharyngeal arch 2 ISH
edn1tf216b/tf216b(TU) standard conditions Prim-15 mandibular arch skeleton ISH
Prim-15 pharyngeal arch 1 ISH
Prim-15 pharyngeal arch 2 ISH
el249Tg ; kca4Tg(TU) heat shock Prim-15 pharyngeal arch 1 ISH
Prim-15 pharyngeal arch 2 ISH
nr2f1b TU standard conditions Prim-15 pharyngeal arch 1 ISH
Prim-15 pharyngeal arch 2 ISH
edn1tf216b/tf216b(TU) standard conditions Prim-15 mandibular arch skeleton ISH
Prim-15 pharyngeal arch 1 ISH
Prim-15 pharyngeal arch 2 ISH
el249Tg ; kca4Tg(TU) heat shock Prim-15 pharyngeal arch 1 ISH
Prim-15 pharyngeal arch 2 ISH
nr2f2 TU standard conditions Prim-15 pharyngeal arch 1 ISH
Prim-15 pharyngeal arch 2 ISH
edn1tf216b/tf216b(TU) standard conditions Prim-15 mandibular arch skeleton ISH
Prim-15 pharyngeal arch 1 ISH
Prim-15 pharyngeal arch 2 ISH
el249Tg ; kca4Tg(TU) heat shock Prim-15 pharyngeal arch 1 ISH
Prim-15 pharyngeal arch 2 ISH
nr2f5 TU standard conditions Prim-15 pharyngeal arch 1 ISH
Prim-15 pharyngeal arch 2 ISH
edn1tf216b/tf216b(TU) standard conditions Prim-15 mandibular arch skeleton ISH
Prim-15 pharyngeal arch 1 ISH
Prim-15 pharyngeal arch 2 ISH
el249Tg ; kca4Tg(TU) heat shock Prim-15 pharyngeal arch 1 ISH
Prim-15 pharyngeal arch 2 ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
edn1tf216b/tf216b(TU) standard conditions Prim-15 mandibular arch skeleton nr2f1a expression increased amount, abnormal
Prim-15 mandibular arch skeleton nr2f1b expression increased amount, abnormal
Prim-15 mandibular arch skeleton nr2f2 expression increased amount, abnormal
Prim-15 mandibular arch skeleton nr2f5 expression increased amount, abnormal
Prim-15 pharyngeal arch 1 nr2f1a expression increased distribution, abnormal
Prim-15 pharyngeal arch 1 nr2f1b expression increased distribution, abnormal
Prim-15 pharyngeal arch 1 nr2f2 expression increased distribution, abnormal
Prim-15 pharyngeal arch 1 nr2f5 expression increased distribution, abnormal
Prim-15 pharyngeal arch 2 nr2f1a expression increased distribution, abnormal
Prim-15 pharyngeal arch 2 nr2f1b expression increased distribution, abnormal
Prim-15 pharyngeal arch 2 nr2f2 expression increased distribution, abnormal
Prim-15 pharyngeal arch 2 nr2f5 expression increased distribution, abnormal
el249Tg ; kca4Tg(TU) heat shock Prim-15 pharyngeal arch 1 nr2f1a expression decreased amount, abnormal
Prim-15 pharyngeal arch 1 nr2f1b expression decreased amount, abnormal
Prim-15 pharyngeal arch 1 nr2f2 expression decreased amount, abnormal
Prim-15 pharyngeal arch 1 nr2f5 expression decreased amount, abnormal
Prim-15 pharyngeal arch 2 nr2f1a expression decreased amount, abnormal
Prim-15 pharyngeal arch 2 nr2f1b expression decreased amount, abnormal
Prim-15 pharyngeal arch 2 nr2f2 expression decreased amount, abnormal
Prim-15 pharyngeal arch 2 nr2f5 expression decreased amount, abnormal
nr2f5el611/el611 ; nr2f1bel523/+ ; nr2f2el506/el506(TU) standard conditions Prim-15 pharyngeal arch 1 ednraa expression decreased amount, abnormal
Prim-15 pharyngeal arch 2 ednraa expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 44(3), Barske, L., Rataud, P., Behizad, K., Del Rio, L., Cox, S.G., Crump, J.G., Essential Role of Nr2f Nuclear Receptors in Patterning the Vertebrate Upper Jaw, 337-347.e5, Copyright (2018) with permission from Elsevier. Full text @ Dev. Cell