Figure 2
- ID
- ZDB-FIG-211120-76
- Publication
- Lai et al., 2021 - MicroRNA-21 Plays Multiple Oncometabolic Roles in Colitis-Associated Carcinoma and Colorectal Cancer via the PI3K/AKT, STAT3, and PDCD4/TNF-α Signaling Pathways in Zebrafish
- Other Figures
- (all 11)
- All Figure Page
- Back to All Figure Page
Identification of the gene markers of intestinal inflammation in zebrafish. (A) Schematic diagram of the dextran sodium sulfate (DSS) treatment strategy. Wild-type (WT) larvae were injected with PBS and 10 ng DSS twice at 14 and 16 days post-fertilization (dpf), and expression of the gene markers of intestinal inflammation was analyzed using RT-qPCR at 21 days post-fertilization (dpf). (B) Survival proportions of injected WT larvae from 0 dpi (days post-injection) to 7 dpi (14 to 21 dpf, p < 0.0001, n = 100 in three independent experiments). The survival rate of DSS-injected WT larvae was approximately 50%. The survival rates were not significantly different between non-injected and PBS-injected WT larvae (>90%). (C,D) RT-qPCR analysis of inflammation-related genes in the intestines of PBS- and DSS-injected WT lines at 21 dpf. mRNA expression levels were normalized to gapdh in the intestine of PBS-injected WT controls. Statistically significant differences from the PBS-injected WT control are denoted by * (p < 0.05), ** (p < 0.01), and *** (p < 0.001) for all panels. |
Genes: | (all 19) |
---|---|
Fish: | |
Condition: | |
Anatomical Term: | |
Stage: | Days 21-29 |
Fish: | |
---|---|
Condition: | |
Observed In: | |
Stage Range: | Days 14-20 to Days 21-29 |