ZFIN ID: ZDB-FIG-140617-16
Flasse et al., 2013 - Ascl1b and Neurod1, instead of Neurog3, control pancreatic endocrine cell fate in zebrafish. BMC Biology   11(1):78 Full text @ BMC Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: 5-9 somites to 14-19 somites

Fig. 3 ascl1b and neurod1 are both expressed in pancreatic endocrine precursors. (A-J) Double fluorescent WISH comparing the expression of ascl1b and neurod1 with the expression of the pancreatic factor sox4b(A, B, F, G), pdx1(D, E) or with the insulin hormone (I, J) at early stages (12 to 16 hpf). The arrows point to a cell neurod1+/sox4b- (G) or neurod1+/ascl1b- (H). The arrowheads point to a cell neurod1+/ascl1b+(H). Z-plane confocal images. All views are ventral with anterior part to the left. (K) Diagram illustrating the time windows for the expression of ascl1b, neurod1 and the pancreatic hormones. Scale bars : 50 ┬Ám hpf, hours post fertilization; WISH, whole-mount in situ hybridization.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ascl1b WT standard conditions 5-9 somites trunk embryonic structure IHC
10-13 somites pancreas primordium IHC
14-19 somites pancreas primordium IHC
ins Ab2-ins WT standard conditions 14-19 somites pancreas primordium IHC
neurod1 WT standard conditions 5-9 somites trunk embryonic structure IHC
10-13 somites pancreas primordium IHC
14-19 somites pancreas primordium IHC
pdx1 WT standard conditions 10-13 somites pancreas primordium IHC
sox4b WT standard conditions 5-9 somites trunk embryonic structure IHC
10-13 somites pancreas primordium IHC
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal BMC Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Biol.