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ZFIN ID: ZDB-FIG-140617-19
Flasse et al., 2013 - Ascl1b and Neurod1, instead of Neurog3, control pancreatic endocrine cell fate in zebrafish. BMC Biology   11(1):78 Full text @ BMC Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage Range: 14-19 somites to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Fig. 6 Pancreatic cells are arrested in their differentiation process in neurod1 morphants and the expression of sox4b is not maintained. Ventral views with the anterior to the left of the pancreas of embryos analyzed by visible (C-H, U-X) or fluorescent (A-B, I-T) WISH. (A-H) Pancreatic expression of mnx1, arx, isl1 and pax6b in controls and Mo2-neurod1 morphants at 24 hpf, the number of isl1+ cells in control morphant (50.4 ± 2.1) was reduced about 2.1 fold in the Mo2-neurod1 morphant (24.1 ± 1.2) and the number of pax6b+ cells in control morphant (40.7 ± 1.2) was reduced about 1.8 fold in the Mo2-neurod1 morphant (22.1 ± 1.2). (I-L) Double fluorescent WISH performed with pax6b(I, J) or isl1(K,L) probe together with a mix of insulin (ins) and somatostatin (sst) probes on 24 hpf controls or Mo2-neurod1 morphants. Z-plane confocal images (M-T) Double fluorescent WISH performed with sox4b and neurod1 on 14 hpf and 18 hpf controls or Mo2-neurod1 morphants. Confocal projection images (U-X) WISH performed with sox4b and neurod1 on 24 hpf controls or Mo2-neurod1 morphants. Scale bars : 50 µm hpf, hours post fertilization; WISH, whole-mount in situ hybridization.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
arxa WT control Prim-5 pancreas primordium ISH
WT + MO3-neurod1 standard conditions Prim-5 Not Detected pancreas primordium ISH
ins Ab2-ins WT standard conditions Prim-5 pancreas primordium IHC
Ab2-ins WT + MO3-neurod1 standard conditions Prim-5 pancreas primordium IHC
isl1a WT control Prim-5 pancreas primordium ISH
WT + MO3-neurod1 standard conditions Prim-5 pancreas primordium ISH
mnx1 WT control Prim-5 pancreas primordium IHC
WT + MO3-neurod1 standard conditions Prim-5 pancreas primordium IHC
neurod1 WT control 14-19 somites pancreas primordium IHC
Prim-5 pancreas primordium ISH
WT + MO3-neurod1 standard conditions 14-19 somites pancreas primordium IHC
Prim-5 pancreas primordium ISH
pax6b WT control Prim-5 pancreas primordium IHC
Prim-5 pancreas primordium ISH
WT + MO3-neurod1 standard conditions Prim-5 pancreas primordium IHC
Prim-5 pancreas primordium ISH
sox4b WT control Prim-5 pancreas primordium ISH
WT standard conditions 14-19 somites pancreas primordium IHC
WT + MO3-neurod1 standard conditions 14-19 somites pancreas primordium IHC
Prim-5 pancreas primordium ISH
sst2 WT control Prim-5 pancreas primordium IHC
WT + MO3-neurod1 standard conditions Prim-5 pancreas primordium IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO3-neurod1 standard conditions Prim-5 endocrine pancreas development disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal BMC Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Biol.