ZFIN ID: ZDB-FIG-140617-18
Flasse et al., 2013 - Ascl1b and Neurod1, instead of Neurog3, control pancreatic endocrine cell fate in zebrafish. BMC Biology   11(1):78 Full text @ BMC Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Prim-15
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-15

Fig. 5 Impaired endocrine cell differentiation in neurod1 morphants. Ventral views with anterior to the left of WISH showing expression of insulin(A-C), somatostatin(D-F), ghrelin(G-I) and glucagon(J-L) in control (A, D, G, J), Mo1-neurod1(B, E, H, K) and Mo2-neurod1(C, F, I, L) morphants at 30 hpf. The quantifications on the right side of the figure represent the number of positive cells per embryo for controls and neurod1 morphants. Asterisks (***) indicate that the difference between cell number in controls and neurod1 morphants is statistically significant by Student’s t-test (P <0.001). Scale bars : 50 µm hpf, hours post fertilization; WISH, whole-mount in situ hybridization.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
gcga WT control Prim-15 pancreatic bud ISH
WT + MO2-neurod1 standard conditions Prim-15 Not Detected pancreatic bud ISH
WT + MO3-neurod1 standard conditions Prim-15 Not Detected pancreatic bud ISH
ghrl WT control Prim-15 pancreatic bud ISH
WT + MO2-neurod1 standard conditions Prim-15 pancreatic bud ISH
WT + MO3-neurod1 standard conditions Prim-15 pancreatic bud ISH
ins WT control Prim-15 pancreatic bud ISH
WT + MO2-neurod1 standard conditions Prim-15 pancreatic bud ISH
WT + MO3-neurod1 standard conditions Prim-15 pancreatic bud ISH
sst2 WT control Prim-15 pancreatic bud ISH
WT + MO2-neurod1 standard conditions Prim-15 pancreatic bud ISH
WT + MO3-neurod1 standard conditions Prim-15 pancreatic bud ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-neurod1 standard conditions Prim-15 endocrine pancreas development disrupted, abnormal
WT + MO3-neurod1 standard conditions Prim-15 endocrine pancreas development disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal BMC Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Biol.